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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Compositional profile of alpha/beta-hydrolase fold proteins in mangrove soil metagenomes: prevalence of epoxide hydrolases and haloalkane dehalogenases in oil-contaminated sites

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Author(s):
Jimenez, Diego Javier [1] ; Dini-Andreote, Francisco [1] ; Ottoni, Julia Ronzella [2] ; de Oliveira, Valeria Maia [2] ; van Elsas, Jan Dirk [1] ; Andreote, Fernando Dini [3]
Total Authors: 6
Affiliation:
[1] Univ Groningen, Ctr Ecol & Evolutionary Studies, Dept Microbial Ecol, NL-9747 AG Groningen - Netherlands
[2] Univ Estadual Campinas, Res Ctr Chem Biol & Agr, Microbial Resources Div, BR-6171 Campinas, SP - Brazil
[3] Univ Sao Paulo, Luiz de Queiroz Coll Agr, Dept Soil Sci, BR-13418900 Piracicaba, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: MICROBIAL BIOTECHNOLOGY; v. 8, n. 3, p. 604-613, MAY 2015.
Web of Science Citations: 7
Abstract

The occurrence of genes encoding biotechnologically relevant /-hydrolases in mangrove soil microbial communities was assessed using data obtained by whole-metagenome sequencing of four mangroves areas, denoted BrMgv01 to BrMgv04, in SAo Paulo, Brazil. The sequences (215Mb in total) were filtered based on local amino acid alignments against the Lipase Engineering Database. In total, 5923 unassembled sequences were affiliated with 30 different /-hydrolase fold superfamilies. The most abundant predicted proteins encompassed cytosolic hydrolases (abH08;approximate to 23%), microsomal hydrolases (abH09;approximate to 12%) and Moraxella lipase-like proteins (abH04 and abH01; <5%). Detailed analysis of the genes predicted to encode proteins of the abH08 superfamily revealed a high proportion related to epoxide hydrolases and haloalkane dehalogenases in polluted mangroves BrMgv01-02-03. This suggested selection and putative involvement in local degradation/detoxification of the pollutants. Seven sequences that were annotated as genes for putative epoxide hydrolases and five for putative haloalkane dehalogenases were found in a fosmid library generated from BrMgv02 DNA. The latter enzymes were predicted to belong to Actinobacteria, Deinococcus-Thermus, Planctomycetes and Proteobacteria. Our integrated approach thus identified 12 genes (complete and/or partial) that may encode hitherto undescribed enzymes. The low amino acid identity (<60%) with already-described genes opens perspectives for both production in an expression host and genetic screening of metagenomes. (AU)

FAPESP's process: 04/13910-6 - Biodiversity and functional activities of microorganisms from mangrove of the State of São Paulo
Grantee:Itamar Soares de Melo
Support type: BIOTA-FAPESP Program - Thematic Grants
FAPESP's process: 12/06245-2 - Metatranscriptomics and genomic context of microbial genes related to biogeochemical cycles in mangroves
Grantee:Fernando Dini Andreote
Support type: Regular Research Grants