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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effect of N-acetylcysteine plus deferoxamine on oxidative stress and inflammation in dystrophic muscle cells

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Rapucci Moraes, Luis Henrique [1] ; Bollineli, Roberta Constancio [1] ; Mizobuti, Daniela Sayuri [1] ; Silveira, Leonardo dos Reis [2, 3] ; Marques, Maria Julia [1] ; Minatel, Elaine [1]
Total Authors: 6
[1] Univ Estadual Campinas, UNICAMP, Inst Biol, Dept Biol Estrutural Func, BR-13083970 Campinas, SP - Brazil
[2] Univ Sao Paulo, FMRP, Dept Bioquim Imunol, Ribeirao Preto, SP - Brazil
[3] Univ Sao Paulo, Escola Educ Fis & Esporte Ribeirao Preto, Ribeirao Preto, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Redox Report; v. 20, n. 3, p. 109-115, MAY 2015.
Web of Science Citations: 6

Objectives: Oxidative stress and inflammatory process play an important role in the pathogenesis of Duchenne muscular dystrophy (DMD). We investigated whether deferoxamine (DFX) improves the antioxidant effects of N-acetylcysteine (NAC) on primary cultures of dystrophic muscle cells from mdx mice, the experimental model of DMD. Methods: Primary cultures of skeletal muscle cells from mdx mice were treated with either NAC (10 mM), DFX (5 mM), or NAC plus DFX for 24 hours. The muscle cells of C57BL/10 mice were used as controls. Results: Production of hydrogen peroxide (H2O2) and levels of 4-hydroxynonenal (4-HNE), tumor necrosis factor alpha (TNF-alpha), and nuclear factor kappa-B (NF-kappa B) were significantly higher in mdx muscle cells than in C57BL/10 muscle cells. Treatment with NAC, DFX, or NAC plus DFX significantly decreased H2O2 production (24, 58, and 72%, respectively), and levels of 4-HNE-protein adducts (62, 33, and 71%, respectively), TNF-alpha (32, 29, and 31%, respectively), and NF-kappa B (34, 38, and 52%, respectively) on dystrophic muscle cells. Discussion: This study demonstrates that mdx muscle cells are able to produce key oxidative stress and inflammatory markers, without the interference of inflammatory cells, and shows that NAC plus DFX reduced the inflammatory and oxidative stress indicators, mainly H2O2 production and NF-kappa B levels by dystrophic fibers. (AU)

FAPESP's process: 10/07775-0 - Muscle cells of mdx and C57BL/10 mice treated with antioxidants and iron chelating
Grantee:Roberta Constancio Bollineli
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 10/01087-4 - In vivo and in vitro treatment, with N-acetylcistein and Deferoxamin in dystrophic mouse
Grantee:Luis Henrique Rapucci Moraes
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 11/02474-4 - Treatment in vivo and in vitro with calcium blocker and antioxidant in mdx mice
Grantee:Elaine Minatel
Support type: Regular Research Grants