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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Multi-Analytical Approach Reveals Potential Microbial Indicators in Soil for Sugarcane Model Systems

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Author(s):
Navarrete, Acacio Aparecido [1] ; Diniz, Tatiana Rosa [1] ; Perez Braga, Lucas Palma [1] ; Silva, Genivaldo Gueiros Zacarias [2] ; Franchini, Julio Cezar [3] ; Rossetto, Raffaella [4] ; Edwards, Robert Alan [5, 6, 2] ; Tsai, Siu Mui [1]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, Ctr Nucl Energy Agr CENA, Cell & Mol Biol Lab, Piracicaba, SP - Brazil
[2] San Diego State Univ, Computat Sci Res Ctr, San Diego, CA 92182 - USA
[3] Brazilian Agr Res Corp, Londrina, PR - Brazil
[4] Sao Paulos Agcy Agribusiness Technol, Piracicaba, SP - Brazil
[5] San Diego State Univ, Dept Comp Sci, San Diego, CA 92182 - USA
[6] Argonne Natl Lab, Div Math & Comp Sci, Argonne, IL 60439 - USA
Total Affiliations: 6
Document type: Journal article
Source: PLoS One; v. 10, n. 6 JUN 9 2015.
Web of Science Citations: 21
Abstract

This study focused on the effects of organic and inorganic amendments and straw retention on the microbial biomass (MB) and taxonomic groups of bacteria in sugarcane-cultivated soils in a greenhouse mesocosm experiment monitored for gas emissions and chemical factors. The experiment consisted of combinations of synthetic nitrogen (N), vinasse (V; a liquid waste from ethanol production), and sugarcane-straw blankets. Increases in CO2-C and N2O-N emissions were identified shortly after the addition of both N and V to the soils, thus increasing MB nitrogen (MB-N) and decreasing MB carbon (MB-C) in the N+V-amended soils and altering soil chemical factors that were correlated with the MB. Across 57 soil metagenomic datasets, Actinobacteria (31.5%), Planctomycetes (12.3%), Deltaproteobacteria (12.3%), Alphaproteobacteria (12.0%) and Betaproteobacteria (11.1%) were the most dominant bacterial groups during the experiment. Differences in relative abundance of metagenomic sequences were mainly revealed for Acidobacteria, Actinobacteria, Gammaproteobacteria and Verrucomicrobia with regard to N+V fertilization and straw retention. Differential abundances in bacterial groups were confirmed using 16S rRNA gene-targeted phylum-specific primers for real-time PCR analysis in all soil samples, whose results were in accordance with sequence data, except for Gammaproteobacteria. Actinobacteria were more responsive to straw retention with Rubrobacterales, Bifidobacteriales and Actinomycetales related to the chemical factors of N+V-amended soils. Acidobacteria subgroup 7 and Opitutae, a verrucomicrobial class, were related to the chemical factors of soils without straw retention as a surface blanket. Taken together, the results showed that MB-C and MB-N responded to changes in soil chemical factors and CO2-C and N2O-N emissions, especially for N+V-amended soils. The results also indicated that several taxonomic groups of bacteria, such as Acidobacteria, Actinobacteria and Verrucomicrobia, and their subgroups acted as early-warning indicators of N+V amendments and straw retention in sugarcane-cultivated soils, which can alter the soil chemical factors. (AU)

FAPESP's process: 11/51749-6 - Functional bioindicators for soil quality monitoring for sustainable management of sugarcane biomass production (BIOSQ)
Grantee:Tsai Siu Mui
Support type: Program for Research on Bioenergy (BIOEN) - Regular Program Grants
FAPESP's process: 12/13321-7 - Taxonomic and functional microbial bio-indicators in soil under sugarcane biomass production systems
Grantee:Acacio Aparecido Navarrete
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 14/06077-8 - Metagenomic annotation and bioinformatics analysis applied to definition of microbial bioindicators for soil agricultural management effects in sugarcane production systems
Grantee:Acacio Aparecido Navarrete
Support type: Scholarships abroad - Research Internship - Post-doctor