| Full text | |
| Author(s): |
Abe, Aneli E.
[1]
;
de Oliveira, Carine E.
[1]
;
Dalboni, Thalita M.
[1]
;
Chagas-Paula, Daniela A.
[2]
;
Rocha, Bruno A.
[2]
;
de Oliveira, Rejane B.
[2]
;
Gasparoto, Thais H.
[1]
;
da Costa, Fernando B.
[2]
;
Campanelli, Ana P.
[1]
Total Authors: 9
|
| Affiliation: | [1] Univ Sao Paulo, Dept Biol Sci, Bauru, SP - Brazil
[2] Univ Sao Paulo, AsterBioChem Res Team, Sch Pharmaceut Sci Ribeirao Preto, Lab Pharmacognosy, Dept Pharmaceut Sci, BR-14049 Ribeirao Preto, SP - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY; v. 25, n. 2, p. 111-116, MAR-APR 2015. |
| Web of Science Citations: | 10 |
| Abstract | |
Abstract The tagitinins isolated of Tithonia diversifolia (Hemsl.) A. Gray, Asteraceae, are the most studied sesquiterpene lactones due to their wide spectrum of pharmacologic activities, especially related with nuclear factor-kappa B inhibition. Nevertheless, detailed studies about the mechanism of action of its active compounds are still lacking. Neutrophils perform a fundamental role in the inflammatory response to several etiologic factors. However, the effect of tagitinins on human neutrophil is not yet clearly known. We investigated the role of tagitinin C (1), tagitinin F (2) and tagitinin A (3) in activation and survival of human neutrophils to establish possible effects in their mechanisms of inflammation. Human neutrophils were purified from the peripheral blood and cultivated with tagitinins C (1), F (2) and A (3) in the presence or not of Escherichia coli lipopolysaccharide. The enzymatic activity, apoptosis and secretion of cytokines rate were determined after 18 h. Lipopolysaccharide-induced myeloperoxidase activity of human neutrophils was significantly inhibited only by tagitinin F (2). Apoptosis of neutrophils was increased in the presence of tagitinin C (1), and it occurred independently of the presence of lipopolysaccharide or dexamethasone. Tagitinins C (1), F (2) and A (3) decrease lipopolysaccharide-induced interleukin-6, interleukin-8 and Tumor necrosis factor alpha production by human neutrophils. Together, these results indicate that tagitinins exhibit anti-inflammatory action on human neutrophils. However, tagitinin F (2) was the only sesquiterpene lactone that decreased secretion of inflammatory products by neutrophils without inducing neutrophil apoptosis. (AU) | |
| FAPESP's process: | 08/02185-0 - Anti-inflammatory activity and phitochemical characterization of tea and differents extracts of Tithonia diversifolia (Asteraceae) |
| Grantee: | Daniela Aparecida Chagas de Paula |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 11/01862-0 - In vivo, in vitro and in silico strategies to assess the structural requirements for the effects of sesquiterpene lactones on seed germination, with emphasis on enzymatic modulation and metabolic profiling of the seeds |
| Grantee: | Rejane Barbosa de Oliveira |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 11/18174-0 - Study of the immunomodulatory activities of Tithonia diversifolia in human neutrophils. |
| Grantee: | Aneli Eiko Abe |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 09/14127-7 - The role of inflammasomes in chemically induced tumor |
| Grantee: | Thaís Helena Gasparoto |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 10/10940-2 - Metabolomic studies of Asteraceae by LC-UV-MS, "in vitro" evaluation of the anti-inflammatory potential and their correlation by "in silico" methods |
| Grantee: | Daniela Aparecida Chagas de Paula |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 10/19317-6 - Activation profile of neutrophils from elderly individuals and expression of IgG receptors |
| Grantee: | Thalita Marcato Dalboni |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |