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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Gene Expression Response of Trichophyton rubrum during Coculture on Keratinocytes Exposed to Antifungal Agents

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Author(s):
Komoto, Tatiana Takahasi [1] ; Bitencourt, Tamires Aparecida [1] ; Silva, Gabriel [1] ; Beleboni, Rene Oliveira [1] ; Marins, Mozart [1] ; Fachin, Ana Lucia [1]
Total Authors: 6
Affiliation:
[1] Univ Ribeirao Preto, Unidade Biotecnol, BR-14096900 Ribeirao Preto, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Evidence-based Complementary and Alternative Medicine; 2015.
Web of Science Citations: 6
Abstract

Trichophyton rubrumis themost common causative agent of dermatomycoses worldwide, causing infection in the stratum corneum, nails, and hair. Despite the high prevalence of these infections, little is known about the molecular mechanisms involved in the fungal-host interaction, particularly during antifungal treatment. The aim of this work was to evaluate the gene expression of T. rubrum cocultured with keratinocytes and treated with the flavonoid trans-chalcone and the glycoalkaloid alpha-solanine. Both substances showed a marked antifungal activity against T. rubrum strain CBS (MIC = 1.15 and 17.8 mu g/mL, resp.). Cytotoxicity assay against HaCaT cells produced IC50 values of 44.18 to trans-chalcone and 61.60 mu M to alpha-solanine. The interaction of keratinocytes with T. rubrum conidia upregulated the expression of genes involved in the glyoxylate cycle, ergosterol synthesis, and genes encoding proteases but downregulated the ABC transporter TruMDR2 gene. However, both antifungals downregulated the ERG1 and ERG11, metalloprotease 4, serine proteinase, and TruMDR2 genes. Furthermore, the trans-chalcone downregulated the genes involved in the glyoxylate pathway, isocitrate lyase, and citrate synthase. Considering the urgent need for more efficient and safer antifungals, these results contribute to a better understanding of fungal-host interactions and to the discovery of new antifungal targets. (AU)

FAPESP's process: 12/03845-9 - Transcriptome of the dermatophyte Trichophyton rubrum in response to antifungal transchalcona in culture conditions that simulate human skin infection
Grantee:Ana Lucia Fachin Saltoratto
Support type: Regular Research Grants
FAPESP's process: 12/02920-7 - Transcriptome of the dermatophyte Trichophyton rubrum in response to antifungal transchalcona in culture conditions that simulate infection
Grantee:Tamires Aparecida Bitencourt
Support type: Scholarships in Brazil - Doctorate