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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Parathyroid Hormone (1-34) Modulates Odontoblast Proliferation and Apoptosis via PKA and PKC-Dependent Pathways

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Author(s):
Guimaraes, Gustavo Narvaes [1] ; Rodrigues, Thaisangela Lopes [2] ; de Souza, Ana Paula [1] ; Line, Sergio Roberto [1] ; Marques, Marcelo Rocha [1]
Total Authors: 5
Affiliation:
[1] Univ Estadual Campinas, Piracicaba Dent Sch, Div Histol & Embryol, Dept Morphol, BR-13414903 Sao Paulo - Brazil
[2] Univ Fed Goias, Sch Dent, Dept Stomatol Sci, Goiania, Go - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Calcified Tissue International; v. 95, n. 3, p. 275-281, SEP 2014.
Web of Science Citations: 4
Abstract

Parathyroid hormone (PTH) plays a key role in the development and homeostasis of mineralized tissues such as bone and dentine. We have reported that PTH (1-34) administration can increase dentine formation in mice and that this hormone modulates in vitro mineralization of odontoblast-like cells. The purpose of the present study was to investigate whether PTH (1-34) participates in the proliferative and apoptotic signaling of odontoblast-like cells (MDPC23). MDPC23 cells were exposed to 50 ng/ml hPTH (1-34) or vehicle for 1 (P1), 24 (P24), or 48 (P48) hours, and the cell proliferation, apoptosis, and cell number were evaluated. To examine whether changes in the proliferative and apoptotic signaling in response to PTH involve protein kinases A (PKA) and/or C (PKC), MDPC23 cells were exposed to PTH with or without PKC or PKA signaling pathway inhibitors. Overall, the results showed that the PKA pathway acts in response to PTH exposure maintaining levels of cell proliferation, while the PKC pathway is mainly involved for longer exposure to PTH (24 or 48 h), leading to the reduction of cell proliferation and increase of apoptosis. The exposure to PTH reduced the cell number in relation to the control group in a time-dependent manner. In conclusion, PTH modulates odontoblast-like cell proliferative and apoptotic response in a time-dependent manner. Both PKC and PKA pathways participate in PTH-induced modulation in an antagonist mode. (AU)

FAPESP's process: 09/06125-4 - Evaluation of the effect of intermittent PTH administration on odontoblast-like cells and dentine formation
Grantee:Marcelo Rocha Marques
Support Opportunities: Regular Research Grants