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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Identification of compounds from high-fat and extra virgin olive oil-supplemented diets in whole mouse liver extracts and isolated mitochondria using mass spectrometry

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dos Santos, Gustavo Aparecido [1] ; Ferreira, Monica Siqueira [1] ; de Oliveira, Diogo Noin [1] ; de Oliveira, Vanessa [2] ; Siqueira-Santos, Edilene S. [3] ; Correa Cintra, Dennys Esper [2] ; Castilho, Roger Frigerio [3] ; Velloso, Licio Augusto [4] ; Catharino, Rodrigo Ramos [1]
Total Authors: 9
[1] Univ Estadual Campinas, Sch Med Sci, Innovare Biomarkers Lab, BR-13083877 Campinas, SP - Brazil
[2] Univ Estadual Campinas, Fac Sci Appl, Sport Sci Course, BR-13083877 Campinas, SP - Brazil
[3] Univ Estadual Campinas, Sch Med Sci, Expt Neurodegenerat Lab, BR-13083877 Campinas, SP - Brazil
[4] Univ Estadual Campinas, Sch Med Sci, Lab Cell Signaling, BR-13083877 Campinas, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Journal of Mass Spectrometry; v. 50, n. 7, p. 951-958, JUL 2015.
Web of Science Citations: 4

Nonalcoholic steatohepatitis (NASH) is a fatty liver disorder that could be improved with extra virgin olive oil (EVOO) supplementation in diet. We propose the monitoring, in whole mouse liver extracts and in isolated mitochondria, of the absorption of compounds from three different diets: standard (CT), high-fat (HFD) and high-fat supplemented with EVOO (HFSO). Male mice were submitted to one of the following three diets: CT or HFD for 16 weeks or HFD for 8 weeks followed by additional 8 weeks with HFSO. Following this period, liver was extracted for histological evaluation, mitochondria isolation and mass spectrometry analyses. Diets, liver extracts and Percoll-purified mitochondria were analyzed using ESI-MS and the lipidomics approach. Morphological, histological and spectrometric results indicated a decrease in NASH severity with EVOO supplementation in comparison with animals maintained with HFD. Spectrometric data also demonstrated that some compounds presented on the diets are absorbed by the mitochondria. EVOO was shown to be a potential therapeutic alternative in food for NASH. Our results are in accordance with the proposition that the major factor that influences different responses to diets is their composition - and not only calories - especially when it comes to studies on obesity. Copyright (C) 2015 John Wiley \& Sons, Ltd. (AU)

FAPESP's process: 14/00084-2 - Identification of adulterations and simultaneous determination of nutrients and contaminants in food using direct analysis mass spectrometry and imaging
Grantee:Diogo Noin de Oliveira
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 11/50400-0 - Mitochondrial energy metabolism, redox state and functionality in cell death and cardiometabolic and neurodegenerative disorders
Grantee:Aníbal Eugênio Vercesi
Support type: Research Projects - Thematic Grants