Lack of acquired resistance in dogs to successive infestations of Rhipicephalus sanguineus ticks from Brazil and Argentina

Author(s)

Evora, Patricia Martinez ^[1] ; Sanches, Gustavo Seron ^[1] ; Gomes Jusi, Marcia Mariza ^[1] ; Rodrigues Alves, Lucas Bocchini ^[1] ; Machado, Rosangela Zacarias ^[1] ; Bechara, Gervasio Henrique ^[1]

Affiliation

^[1] Sao Paulo State Univ UNESP, Fac Agron & Vet Sci FCAV, Dept Vet Pathol, BR-14884900 Sao Paulo - Brazil

• Document type: Journal article
• Source: Experimental and Applied Acarology; v. 67, n. 1, p. 135-146, SEP 2015.
• Web of Science Citations: 2

Abstract

Comparative studies between brown dog tick Rhipicephalus sanguineus populations from Brazil (Jaboticabal, So Paulo) and Argentina (Rafaela, Santa F,) showed significant biological, morphological and genetic differences between them. This work aimed to study, in a comparative way, the acquisition of resistance in domestic dogs to R. sanguineus from Jaboticabal and Rafaela, after successive and controlled infestations. Ticks were kept in a BOD incubator under controlled conditions (27 A degrees C, 80 % relative humidity, 12-h photoperiod). Ten dogs, Dachshund breed, males and females, 6 months old, short- or long-haired, without prior contact with ticks, were used as hosts. They were distributed into two experimental groups composed of five animals each: G1 infested with ten adult couples of R. sanguineus (Jaboticabal) per animal, and G2 infested with ten adult couples of R. sanguineus (Rafaela) per animal. Ticks' biological parameters and titration of antibodies from the dogs' sera by ELISA test were used for comparison between the strains. Results of the biological parameters showed that the dogs did not acquire immunity to either of the R. sanguineus strains after repeated infestations. The ELISA test showed low antibody titers in sera of dogs from G2, in successive infestations, and higher antibody responses post second and third infestations in G1. It also demonstrated cross-reactivity between sera of dogs infested with R. sanguineus (Jaboticabal) and antigens from R. sanguineus (Rafaela) and vice versa. We conclude that Dachshund dogs did not develop resistance against neither Jaboticabal nor Rafaela strains of R. sanguineus. (AU)

• FAPESP's process: 09/13234-4 - Comparative study on the acquisition of resistance in domestic dogs in two strains (Brazil and Argentina) of Rhipicephalus sanguineus (Acari: Ixodidae) following successive and controlled infestations
• Grantee: Patricia Martinez Évora
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 13/09792-7 - EVALUATION OF THE IMMUNOGENIC POTENTIAL OF RHIPICEPHALUS (BOOPHILUS) MICROPLUS AQUAPORIN AGAINST THE RHIPICEPHALUS SANGUINEUS TICK, LATREILLE, 1806 IN DOMESTIC DOGS
• Grantee: Patricia Martinez Évora
• Support Opportunities: Scholarships in Brazil - Doctorate