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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Distribution of cortical granules and meiotic maturation of canine oocytes in bi-phasic systems

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Author(s):
Apparicio, Maricy [1] ; Mostachio, Giuliano Q. [1] ; Motheo, Tathiana F. [1] ; Alves, Aracelle E. [1] ; Padilha, Luciana [1] ; Pires-Butler, Eliandra A. [1] ; Savi, Paula A. P. [1] ; Uscategui, Ricardo A. R. [1] ; Luvoni, Gaia C. [2] ; Vicente, Wilter R. R. [1]
Total Authors: 10
Affiliation:
[1] Univ Estadual Paulista, UNESP, Dept Med Vet Prevent & Reprod Anim, BR-14884900 Jaboticabal, SP - Brazil
[2] Univ Milan, Dipartimento Sci Vet Salute Prod Anim & Sicurezza, I-20133 Milan - Italy
Total Affiliations: 2
Document type: Journal article
Source: REPRODUCTION FERTILITY AND DEVELOPMENT; v. 27, n. 7, p. 1082-1087, 2015.
Web of Science Citations: 4
Abstract

The aim of this study was to evaluate the influence of different bi-phasic systems with gonadotrophins and steroids on in vitro maturation rates of oocytes obtained from bitches at different reproductive stages (follicular, luteal, anoestrous). In System A (control) oocytes were matured for 72h in base medium (BM) with 10IUmL(-1) human chorionic gonadotrophin (hCG), 1gmL(-1) progesterone (P4) and 1gmL(-1) oestradiol (E2); in bi-phasic System B oocytes were matured for 48h in BM with hCG and for 24h in BM with P4; in bi-phasic System C oocytes were matured for 48h in BM with hCG, P4 and E2, and for 24h in BM with P4; in System D, oocytes were cultured in BM without hormonal supplementation. Data were analysed by ANOVA. There was a positive effect of the bi-phasic systems on germinal vesicle breakdown, metaphase I and metaphase II rates, irrespective of reproductive status (P<0.05). Bi-phasic systems were also beneficial for cortical granule distribution (an indication of cytoplasmic maturation) and its relationship to nuclear status: 74.5% of the oocytes cultured in System B and 85.4% of those cultured in System C presented both nuclear and cytoplasmic maturation (P<0.001). The stage of the oestrous cycle did not influence maturation rates. (AU)