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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Impact of Cardiopulmonary Bypass on Respiratory Mucociliary Function in an Experimental Porcine Model

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Author(s):
Sanchez-Veliz, Rodrigo [1] ; Carmona, Maria Jose [1] ; Otsuki, Denise Aya [1] ; Freitas, Claudia [1] ; Benicio, Anderson [2] ; Negri, Elnara Marcia [3] ; Malbouisson, Luiz Marcelo [1]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Sch Med, Lab Anesthesiol LIM08, Sao Paulo - Brazil
[2] Univ Sao Paulo, Sch Med, Cardiac Surg Div, Heart Inst InCor, Sao Paulo - Brazil
[3] Univ Sao Paulo, Sch Med, Expt Air Pollut Lab, Dept Pathol, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: PLoS One; v. 10, n. 8 AUG 19 2015.
Web of Science Citations: 2
Abstract

Background The impact of cardiac surgery using cardiopulmonary bypass (CPB) on the respiratory mucociliary function is unknown. This study evaluated the effects of CPB and interruption of mechanical ventilation on the respiratory mucociliary system. Methods Twenty-two pigs were randomly assigned to the control (n = 10) or CPB group (n = 12). After the induction of anesthesia, a tracheostomy was performed, and tracheal tissue samples were excised (T0) from both groups. All animals underwent thoracotomy. In the CPB group, an aorto-bicaval CPB was installed and maintained for 90 minutes. During the CPB, mechanical ventilation was interrupted, and the tracheal tube was disconnected. A second tracheal tissue sample was obtained 180 minutes after the tracheostomy (T180). Mucus samples were collected from the trachea using a bronchoscope at T0, T90 and T180. Ciliary beat frequency (CBF) and in situ mucociliary transport (MCT) were studied in ex vivo tracheal epithelium. Mucus viscosity (MV) was assessed using a cone-plate viscometer. Qualitative tracheal histological analysis was performed at T180 tissue samples. Results CBF decreased in the CPB group (13.1 +/- 1.9 Hz vs. 11.1 +/- 2.1 Hz, p < 0.05) but not in the control group (13.1 +/- 1 Hz vs. 13 +/- 2.9 Hz). At T90, viscosity was increased in the CPB group compared to the control (p < 0.05). No significant differences were observed in in situ MCT. Tracheal histology in the CPB group showed areas of ciliated epithelium loss, submucosal edema and infiltration of inflammatory cells. Conclusion CPB acutely contributed to alterations in tracheal mucocilliary function. (AU)