| Full text | |
| Author(s): Show less - |
Bufalino, Andreia
[1]
;
Cervigne, Nilva K.
[1]
;
de Oliveira, Carine Ervolino
[1]
;
Fonseca, Felipe Paiva
[1]
;
Rodrigues, Priscila Campioni
[1]
;
Soares Macedo, Carolina Carneiro
[1]
;
Sobral, Lays Martin
[1]
;
Miguel, Marcia Costa
[2, 1]
;
Lopes, Marcio Ajudarte
[1]
;
Paes Leme, Adriana Franco
[3]
;
Lambert, Daniel W.
[4, 5]
;
Salo, Tuula A.
[6, 7, 8, 9, 1]
;
Kowalski, Luiz Paulo
[10]
;
Graner, Edgard
[1]
;
Coletta, Ricardo D.
[1]
Total Authors: 15
|
| Affiliation: | [1] Univ Estadual Campinas, Sch Dent, Dept Oral Diag, Piracicaba, SP - Brazil
[2] Univ Fed Rio Grande do Norte, Dept Dent, BR-59072970 Natal, RN - Brazil
[3] CNPEM, Brazilian Biociences Natl Lab, Campinas, SP - Brazil
[4] Univ Sheffield, Sch Clin Dent, Integrated Biosci, Sheffield, S Yorkshire - England
[5] Univ Sheffield, Sheffield Canc Ctr, Sheffield, S Yorkshire - England
[6] Univ Oulu, Oulu Univ Hosp, Oulu - Finland
[7] Univ Oulu, Med Res Ctr, Oulu - Finland
[8] Univ Helsinki, Inst Dent, Helsinki - Finland
[9] Univ Oulu, Inst Dent, Dept Diagnost & Oral Med, Oulu - Finland
[10] AC Camargo Canc Ctr, Dept Head & Neck Surg & Otorhinolaryngol, Sao Paulo, SP - Brazil
Total Affiliations: 10
|
| Document type: | Journal article |
| Source: | PLoS One; v. 10, n. 8 AUG 28 2015. |
| Web of Science Citations: | 20 |
| Abstract | |
Deregulated expression of activin A is reported in several tumors, but its biological functions in oral squamous cell carcinoma (OSCC) are unknown. Here, we investigate whether activin A can play a causal role in OSCCs. Activin A expression was assessed by qPCR and immunohistochemistry in OSCC tissues. Low activin A-expressing cells were treated with recombinant activin A and assessed for apoptosis, proliferation, adhesion, migration, invasion and epithelial-mesenchymal transition (EMT). Those phenotypes were also evaluated in high activin A-expressing cells treated with follistatin (an activin A antagonist) or stably expressing shRNA targeting activin A. Transfections of microRNA mimics were performed to determine whether the overexpression of activin A is regulated by miR-143/miR-145 cluster. Activin A was overexpressed in OSCCs in comparison with normal oral mucosa, and high activin A levels were significantly associated with lymph node metastasis, tumor differentiation and poor survival. High activin A levels promoted multiple properties associated with malignant transformation, including decreased apoptosis and increased proliferation, migration, invasion and EMT. Both miR-143 and miR-145 were markedly downregulated in OSCC cell lines and in clinical specimens, and inversely correlated to activin A levels. Forced expression of miR-143 and miR-145 in OSCC cells significantly decreased the expression of activin A. Overexpression of activin A in OSCCs, which is controlled by downregulation of miR-143/miR-145 cluster, regulates apoptosis, proliferation and invasiveness, and it is clinically correlated with lymph node metastasis and poor survival. (AU) | |
| FAPESP's process: | 13/01607-6 - The biological role of EEF1D, fascin and plectin on oral squamous cell carcinoma |
| Grantee: | Ricardo Della Coletta |
| Support Opportunities: | Regular Research Grants |