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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Topical Skin Cancer Therapy Using Doxorubicin-Loaded Cationic Lipid Nanoparticles and Iontophoresis

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Huber, Lucas A. [1] ; Pereira, Tatiana A. [1] ; Ramos, Danielle N. [1] ; Rezende, Lucas C. D. [1] ; Emery, Flavio S. [1] ; Sobral, Lays Martin [1] ; Leopoldino, Andreia Machado [1] ; Lopez, Renata F. V. [1]
Total Authors: 8
[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: JOURNAL OF BIOMEDICAL NANOTECHNOLOGY; v. 11, n. 11, p. 1975-1988, NOV 2015.
Web of Science Citations: 24

The topical administration of chemotherapeutics is a promising approach for the treatment of skin cancer; however, different pharmaceutical strategies are required to allow large amounts of drug to penetrate tumors. This work examined the potential of the anodic iontophoresis of doxorubicin-loaded cationic solid lipid nanoparticles (DOX-SLN) to increase the distribution and tumor penetration of DOX. A double-labeled cationic DOX-SLN composed of the lipids stearic acid and monoolein and anew BODIPY dye was prepared and characterized. The skin distribution and penetration of DOX were evaluated in vitro using confocal microscopy and vertical diffusion cells, respectively. The antitumor potential was evaluated in vivo through the anodic iontophoresis of DOX-SLN in squamous cell carcinoma induced in nude BALB/c mice. The encapsulation of DOX drastically altered the DOX partition coefficient and increased the distribution of DOX in the lipid matrix of the stratum corneum (SC). The association with iontophoresis created high-concentration drug reservoir zones in the follicles of the skin. Although the iontophoresis of a DOX solution increased the penetration of DOX in the viable epidermis by approximately 4-fold, the iontophoresis of cationic DOX-SLN increased the DOX penetration by approximately 50-fold. In vivo, the DOX-SLN iontophoretic treatment was effective in inhibiting tumor cell survival and. tumor growth and was accompanied by an increase in keratinization and consequent cell death. These results indicate a strong and synergic effect of iontophoresis with DOX-SLN and provide a potential strategy for the treatment of skin cancer. (AU)

FAPESP's process: 11/06202-9 - Influence of low frequency sonophoresis on skin penetration of nanoparticles aiming the skin cancer treatment
Grantee:Renata Fonseca Vianna Lopez
Support type: Regular Research Grants
FAPESP's process: 10/20794-3 - Influence of low frequency ultrasound (LFU) associated with different coupling medium in skin permeability and topical skin cancer treatment
Grantee:Tatiana Aparecida Pereira
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 11/04384-2 - Influence of iontophoresis on the penetration of solid lipid nanoparticles in skin tumors
Grantee:Lucas de Andrade Huber
Support type: Scholarships in Brazil - Master