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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

References genes for qRT-PCR in guarana (Paullina cupana var. sorbilis)

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Schimpl, Flavia Camila [1] ; Domingues Junior, Adilson Pereira [1] ; de Carvalho Goncalves, Jose Francisco [2] ; da Silva, Jose Ferreira [3] ; Mazzafera, Paulo [1]
Total Authors: 5
[1] Univ Estadual Campinas, Dept Biol Vegetal IB, BR-13083970 Sao Paulo - Brazil
[2] Inst Nacl de Pesquisas da Amazonia, Manaus, Amazonas - Brazil
[3] Univ Fed Amazonas, Fac Ciencias Agr, Dept Prod Anim & Vegetal, Manaus, Amazonas - Brazil
Total Affiliations: 3
Document type: Journal article
Source: BRAZILIAN JOURNAL OF BOTANY; v. 38, n. 2, p. 281-288, JUN 2015.
Web of Science Citations: 1

Gene expression has been extensively studied in plant science research, mainly for the assessment of plant stress responses. Real-time-quantitative polymerase chain reaction (RT-qPCR) is an important tool for obtaining this information because it is a quick and easy technique to acquire a large amount of molecular data for both model and non-model plants. For a successful RT-qPCR analysis, gene expression should be carefully normalised. Genes involved in essential biological processes that exhibit constitutive expression are commonly selected as internal standards to normalise RT-qPCR experiments. In this study, the transcription profiles of 13 candidate reference genes for RT-qPCR were evaluated in three guarana cultivars (BRS-Amazonas, BRS-Maues and BRS-Luzeia) using different tissues (vegetative and fruit) in varying developmental stages. Two different algorithms, NormFinder and GeNorm, were utilised to assess gene stability. In general, the two algorithms did not select the same pairs of genes for all analysed conditions. For the largest group (the fruits of all cultivars), NormFinder selected the pair EF1A/UBQ, whereas GeNorm chose ACT/GAPDH as the best normalising genes. Thus, we recommend the use of at least four reference genes for the normalisation of gene expression in guarana plant studies. (AU)

FAPESP's process: 11/03266-6 - Analysis of the expression of the gene of caffeine synthase in guaraná fruits (Paullina cupana var. sorbilis)
Grantee:Flávia Camila Schimpl
Support type: Scholarships in Brazil - Master