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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effective protection of biological membranes against photo-oxidative damage: Polymeric antioxidant forming a protecting shield over the membrane

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Mertins, Omar [1, 2] ; Mathews, Patrick D. [1] ; Gomide, Andreza B. [2] ; Baptista, Mauricio S. [3] ; Itri, Rosangela [2]
Total Authors: 5
[1] Univ Fed Sao Paulo, Dept Biophys, Escola Paulista Med, BR-04023062 Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Phys, Dept Appl Phys, BR-05508900 Sao Paulo - Brazil
[3] Univ Sao Paulo, Inst Chem, Dept Biochem, BR-05508900 Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES; v. 1848, n. 10, A, p. 2180-2187, OCT 2015.
Web of Science Citations: 7

We have prepared a chitosan polymer modified with gallic acid in order to develop an efficient protection strategy biological membranes against photodamage. Lipid bilayers were challenged with photoinduced damage by photosensitization with methylene blue, which usually causes formation of hydroperoxides, increasing area per lipid, and afterwards allowing leakage of internal materials. The damage was delayed by a solution of gallic acid in a concentration dependent manner, but further suppressed by the polymer at very low concentrations. The membrane of giant unilamellar vesicles was covered with this modified macromolecule leading to a powerful shield against singlet oxygen and thus effectively protecting the lipid membrane from oxidative stress. The results have proven the discovery of a promising strategy for photo protection of biological membranes. (C) 2015 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 10/18163-5 - Membrane photo-protection as investigated by optical microscopy on giant unilamellar vesicles
Grantee:Omar Mertins
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 12/50680-5 - Photosensitization in life sciences
Grantee:Mauricio da Silva Baptista
Support type: Research Projects - Thematic Grants