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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

In-Vitro Antiviral Activities of Extracts of Plants of The Brazilian Cerrado against the Avian Metapneumovirus (aMPV)

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Kohn, L. K. [1, 2] ; Foglio, M. A. [3] ; Rodrigues, R. A. [3] ; Sousa, I. M. de O. [3] ; Martini, M. C. [1] ; Padilla, M. A. [1] ; de Lima Neto, D. F. [1] ; Arns, C. W. [1]
Total Authors: 8
[1] Univ Campinas Unicamp, Inst Biol, Virol Lab, BR-13083970 Campinas, SP - Brazil
[2] Univ Sao Francisco, BR-13045510 Campinas, SP - Brazil
[3] Univ Campinas Unicamp, Inst Chem, Lab Res & Dev Nat Prod, BR-13083970 Campinas, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Brazilian Journal of Poultry Science; v. 17, n. 3, p. 275-280, JUL-SEP 2015.
Web of Science Citations: 3

Avian metapneumovirus (aMPV) is a negative-sense single-stranded RNA enveloped virus of the Metapneumovirus genus belonging to the Paramyxoviridae family. This virus may cause significant economic losses to the poultry industry, despite vaccination, which is the main tool for controlling and preventing aMPV. The aim of this study was to evaluate the antiviral activity of extracts of four different native plants of the Brazilian Cerrado against aMPV. The antiviral activity against aMPV was determined by titration. This technique measures the ability of plant extract dilutions (25 to 2.5 mu g mL(-1)) to inhibit the cytopathic effect (CPE) of the virus, expressed as inhibition percentage (IP). The maximum nontoxic concentration (MNTC) of the extracts used in antiviral assay was 25 mu g mL(-1) for Aspidosperma tomentosum and Gaylussacia brasiliensis, and 2.5 mu g mL(-1) for Arrabidaea chica and Virola sebifera. Twelve different extracts derived from four plant species collected from the Brazilian Cerrado were screened for antiviral activity against aMPV. G. brasiliensis, A. chica, and V. sebifera extracts presented inhibition rates of 99% in the early viral replication stages, suggesting that these extracts act during the adsorption phase. On the other hand, A. tomentosum inhibited 99% virus replication after the virus entered the cell. The biomonitored fractioning of extracts active against aMPV may be a tool to identify the active compounds of plant extracts and to determine their precise mode of action. (AU)

FAPESP's process: 08/00331-9 - Use of microbial co-cultures towards the production and discovery of bioactive natural products
Grantee:Miriam Harumi Kossuga
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 08/00700-4 - Implantation and screening of the antiviral activity from brasilian plants crude extract front the animal virus
Grantee:Clarice Weis Arns
Support type: Regular Research Grants