Molecular studies in neoplasias of children and adolescents: microRNAs signature a...
| Full text | |
| Author(s): |
Kaid, Carolini
[1]
;
Silva, Patricia B. G.
[1]
;
Cortez, Beatriz A.
[1]
;
Rodini, Carolina O.
[1]
;
Semedo-Kuriki, Patricia
[1]
;
Okamoto, Oswaldo K.
[1]
Total Authors: 6
|
| Affiliation: | [1] Univ Sao Paulo, Biosci Inst, Human Genome & Stem Cell Res Ctr, Dept Genet & Evolutionary Biol, BR-05508090 Sao Paulo, SP - Brazil
Total Affiliations: 1
|
| Document type: | Journal article |
| Source: | Cancer Science; v. 106, n. 9, p. 1188-1195, SEP 2015. |
| Web of Science Citations: | 23 |
| Abstract | |
In medulloblastoma, abnormal expression of pluripotency factors such as LIN28 and OCT4 has been correlated with poor patient survival. The miR-302/367 cluster has also been shown to control self-renewal and pluripotency in human embryonic stem cells and induced pluripotent stem cells, but there is limited, mostly correlational, information about these pluripotency-related miRNA in cancer. We evaluated whether aberrant expression of such miRNA could affect tumor cell behavior and stem-like traits, thereby contributing to the aggressiveness of medulloblastoma cells. Basal expression of primary and mature forms of miR-367 were detected in four human medulloblastoma cell lines and expression of the latter was found to be upregulated upon enforced expression of OCT4A. Transient overexpression of miR-367 significantly enhanced tumor features typically correlated with poor prognosis; namely, cell proliferation, 3-D tumor spheroid cell invasion and the ability to generate neurosphere-like structures enriched in CD133 expressing cells. A concurrent downregulation of the miR-367 cancer-related targets RYR3, ITGAV and RAB23, was also detected in miR-367-overexpressing cells. Overall, these findings support the pro-oncogenic activity of miR-367 in medulloblastoma and reveal a possible mechanism contributing to tumor aggressiveness, which could be further explored to improve patient stratification and treatment of this important type of pediatric brain cancer. (AU) | |
| FAPESP's process: | 13/08028-1 - CEGH-CEL - Human Genome and Stem Cell Research Center |
| Grantee: | Mayana Zatz |
| Support type: | Research Grants - Research, Innovation and Dissemination Centers - RIDC |
| FAPESP's process: | 14/10519-6 - Hippo signaling pathway and asymmetric division of cancer stem cells derived from human medulloblastoma |
| Grantee: | Beatriz de Araujo Cortez |
| Support type: | Scholarships in Brazil - Post-Doctorate |
| FAPESP's process: | 13/17566-7 - Pluripotency factor OCT4A and aggressiveness of human medulloblastoma |
| Grantee: | Patrícia Benites Gonçalves da Silva |
| Support type: | Scholarships in Brazil - Doctorate (Direct) |
| FAPESP's process: | 13/02983-1 - Expression of hsa-miR-367 and aggressiveness of human medulloblastoma |
| Grantee: | Carolini Kaid Dávila |
| Support type: | Scholarships in Brazil - Master |
| FAPESP's process: | 11/10001-9 - Contribution of mesenchymal stem cells to tumor development |
| Grantee: | Carolina de Oliveira Rodini |
| Support type: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 10/52686-5 - E2F2 transcription factor and expression of proto-oncogenes in human embryonic stem cells |
| Grantee: | Oswaldo Keith Okamoto |
| Support type: | Regular Research Grants |