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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

miR-367 promotes proliferation and stem-like traits in medulloblastoma cells

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Kaid, Carolini [1] ; Silva, Patricia B. G. [1] ; Cortez, Beatriz A. [1] ; Rodini, Carolina O. [1] ; Semedo-Kuriki, Patricia [1] ; Okamoto, Oswaldo K. [1]
Total Authors: 6
[1] Univ Sao Paulo, Biosci Inst, Human Genome & Stem Cell Res Ctr, Dept Genet & Evolutionary Biol, BR-05508090 Sao Paulo, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Cancer Science; v. 106, n. 9, p. 1188-1195, SEP 2015.
Web of Science Citations: 23

In medulloblastoma, abnormal expression of pluripotency factors such as LIN28 and OCT4 has been correlated with poor patient survival. The miR-302/367 cluster has also been shown to control self-renewal and pluripotency in human embryonic stem cells and induced pluripotent stem cells, but there is limited, mostly correlational, information about these pluripotency-related miRNA in cancer. We evaluated whether aberrant expression of such miRNA could affect tumor cell behavior and stem-like traits, thereby contributing to the aggressiveness of medulloblastoma cells. Basal expression of primary and mature forms of miR-367 were detected in four human medulloblastoma cell lines and expression of the latter was found to be upregulated upon enforced expression of OCT4A. Transient overexpression of miR-367 significantly enhanced tumor features typically correlated with poor prognosis; namely, cell proliferation, 3-D tumor spheroid cell invasion and the ability to generate neurosphere-like structures enriched in CD133 expressing cells. A concurrent downregulation of the miR-367 cancer-related targets RYR3, ITGAV and RAB23, was also detected in miR-367-overexpressing cells. Overall, these findings support the pro-oncogenic activity of miR-367 in medulloblastoma and reveal a possible mechanism contributing to tumor aggressiveness, which could be further explored to improve patient stratification and treatment of this important type of pediatric brain cancer. (AU)

FAPESP's process: 11/10001-9 - Contribution of mesenchymal stem cells to tumor development
Grantee:Carolina de Oliveira Rodini
Support type: Scholarships in Brazil - Doctorate
Grantee:Beatriz de Araujo Cortez
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 13/17566-7 - Pluripotency factor OCT4A and aggressiveness of human medulloblastoma
Grantee:Patrícia Benites Gonçalves da Silva
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 10/52686-5 - E2F2 transcription factor and expression of proto-oncogenes in human embryonic stem cells
Grantee:Oswaldo Keith Okamoto
Support type: Regular Research Grants
FAPESP's process: 13/02983-1 - Expression of hsa-miR-367 and aggressiveness of human medulloblastoma
Grantee:Carolini Kaid Dávila
Support type: Scholarships in Brazil - Master
FAPESP's process: 13/08028-1 - CEGH-CEL - Human Genome and Stem Cell Research Center
Grantee:Mayana Zatz
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC