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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Characterization of the TolB-Pal trans-envelope complex from Xylella fastidiosa reveals a dynamic and coordinated protein expression profile during the biofilm development process

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Author(s):
Santos, Clelton A. [1] ; Janissen, Richard [2] ; Toledo, Marcelo A. S. [1] ; Beloti, Lilian L. [1] ; Azzoni, Adriano R. [3] ; Cotta, Monica A. [2] ; Souza, Mete P. [1, 4]
Total Authors: 7
Affiliation:
[1] Univ Estadual Campinas, Ctr Biol Mol & Engn Genet, BR-13083875 Campinas, SP - Brazil
[2] Univ Estadual Campinas, Inst Fis Gleb Wataghin, BR-13083875 Campinas, SP - Brazil
[3] Univ Sao Paulo, Escola Politecn, Dept Engn Quim, Sao Paulo, SP - Brazil
[4] Univ Estadual Campinas, Inst Biol, Dept Biol Vegetal, BR-13083875 Campinas, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS; v. 1854, n. 10, A, p. 1372-1381, OCT 2015.
Web of Science Citations: 4
Abstract

The intriguing roles of the bacterial Tol-Pal trans-envelope protein complex range from maintenance of cell envelope integrity to potential participation in the process of cell division. In this study, we report the characterization of the XfFolB and XfPal proteins of the Tol-Pal complex of Xylella fastidiosa. X. fastidiosa is a major plant pathogen that forms biofilms inside xylem vessels, triggering the development of diseases in important cultivable plants around the word. Based on functional complementation experiments in Escherichia coli tolB and pal mutant strains, we confirmed the role of xftolB and xfpal in outer membrane integrity. In addition, we observed a dynamic and coordinated protein expression profile during the X. fastidiosa biofilm development process. Using small-angle X-ray scattering (SAXS), the low-resolution structure of the isolated XfToIB-XfPal complex in solution was solved for the first time. Finally, the localization of the XfFolB and XfPal polar ends was visualized via immunofluorescence labeling in vivo during bacterial cell growth. Our results highlight the major role of the components of the cell envelope, particularly the TolB-Pal complex, during the different phases of bacterial biofilm development (C) 2015 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 12/51580-4 - Molecular and functional characterization of proteins potentially related to the phytopatogenicity of biofilm-forming bacteria Xylella fastidiosa
Grantee:Anete Pereira de Souza
Support type: Regular Research Grants