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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Selenium fractionation from plasma, muscle and liver of Nile tilapia (Oreochromis niloticus)

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Author(s):
Silva, Fabio A. [1] ; Cavecci, Bruna [1] ; Baldassini, Welder A. [1] ; Lima, Paula M. [1] ; Moraes, Paula M. [2] ; Roldan, Paulo S. [3] ; Padilha, Cilene C. F. [2] ; Padilha, Pedro M. [2, 4]
Total Authors: 8
Affiliation:
[1] UNESP Sao Paulo State Univ, Fac Vet Med & Anim Sci, Botucatu, SP - Brazil
[2] UNESP Sao Paulo State Univ, Bioci Inst, Botucatu, SP - Brazil
[3] Fed Univ Alagoas IQB UFAL, Inst Chem & Biotechnol, Maceio, AL - Brazil
[4] Univ Estadual Campinas, Natl Inst Bioanalyt Sci & Technol, Campinas, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION; v. 7, n. 4, p. 158-165, DEC 2013.
Web of Science Citations: 4
Abstract

This paper presents the results obtained from selenium fractionation in plasma, muscle and liver samples of Nile tilapia's (Oreochromis niloticus) after protein separation. The plasma, muscle and liver proteome was obtained by 2D-PAGE, and selenium in protein spots was qualitatively and quantitatively determined by synchrotron radiation X-ray fluorescence and graphite furnace atomic absorption spectrometry (GFAAS). The fluorescence spectra indicated the presence of selenium in three protein spots of plasma, two of muscle and one of liver. Selenium was found to be distributed mainly in proteins with a molar mass smaller than 57.0 kDa and with pI in the range of 5.9-9.6, with one exception in the plasma sample, which presented protein with a molar mass of 60.0 kDa. After acid mineralization of the protein spots, a GFAAS determination of the concentration of selenium bound to these proteins indicated a range of 1.35-6.82 mg per g of protein. (AU)

FAPESP's process: 07/59778-0 - Development of analytical methods for evaluation of Nile tilapia (Oreochromis niloticus) metalloproteins
Grantee:Pedro de Magalhães Padilha
Support type: Regular Research Grants