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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Dependence of cytokine levels on the sex of experimental animals: a pilot study on the effect of oestrogen in the temporomandibular joint synovial tissues

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Author(s):
Figueroba, S. R. [1] ; Franco, G. C. N. [2] ; Omar, N. F. [3] ; Groppo, M. F. [3] ; Groppo, F. C. [1]
Total Authors: 5
Affiliation:
[1] Univ Estadual Campinas, UNICAMP, Piracicaba Dent Sch, Dept Physiol Sci, BR-13414903 Sao Paulo - Brazil
[2] Univ Estadual Ponta Grossa, Dept Gen Biol, Area Physiol, Pathophysiol, Ponta Grossa, Parana - Brazil
[3] Univ Estadual Campinas, UNICAMP, Piracicaba Dent Sch, Dept Morphol, BR-13414903 Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: International Journal of Oral and Maxillofacial Surgery; v. 44, n. 11, p. 1368-1375, NOV 2015.
Web of Science Citations: 4
Abstract

The aim of this study was to evaluate the effects of 17 beta-oestradiol (E2) on cartilage thickness and cytokine levels in the temporomandibular joint (TMJ). Thirty rats (15 female, 15 male) were orchidectomized (ORX), ovariectomized (OVX), or sham-operated. After 21 days, animals were assigned to six groups: (1) sham-ORX; (2) ORX; (3) ORX+E2; (4) sham-OVX; (5) OVX; and (6) OVX+E2. Treatments were administered daily for 21 days. The thickness of cartilage layers (fibrous, proliferative, maturation, and hypertrophic) and cytokine levels (interleukins IL-1 alpha, IL-1 beta, IL-6, and tumour necrosis factor alpha (TNF-alpha)) were measured by histomorphometry and ELISA, respectively. Kruskal-Wallis/Durm's tests were used (alpha = 5%). Sham-ORX showed thicker layers than ORX+E2, but not thicker than ORX. All layers, except the hypertrophic layer, were thicker in sham-OVX than OVX or OVX+E2. Although IL-1 beta levels were higher in castrated animals, E2 did not affect the level of this cytokine. IL-1 alpha levels were higher in both ORX (P = 0.0010) and ORX+E2 (P = 0.0053) than in sham-ORX. However, E2 decreased IL-1 alpha levels in OVX (P = 0.0129). When compared to sham-ORX/OVX, IL-6 levels were not affected by E2 in males but were reduced in OVX (P = 0.0079) and increased in OVX+E2 (P = 0.0434). Levels of TNF-alpha were reduced by E2 in both ORX+E2 and OVX+E2. E2 treatment caused gender- and layer-dependent changes in the cartilage. Castration increased all cytokine levels, except for IL-6, without respect to gender. (AU)

FAPESP's process: 06/05023-5 - Influence of hormone replacement and of anti-inflammatory on morphologic parameters, histochemical, and immunohistochemical in rats with or without induced TMD
Grantee:Francisco Carlos Groppo
Support Opportunities: Regular Research Grants