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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Actinobacteria from Termite Mounds Show Antiviral Activity against Bovine Viral Diarrhea Virus, a Surrogate Model for Hepatitis C Virus

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Author(s):
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Padilla, Marina Aiello [1] ; Ferreira Rodrigues, Rodney Alexandre [2] ; Santiago Bastos, Juliana Cristina [1] ; Martini, Matheus Cavalheiro [1] ; de Souza Barnabe, Ana Caroline [1] ; Kohn, Luciana Konecny [1] ; Trovatti Uetanabaro, Ana Paula [3] ; Bomfim, Getulio Freitas [4] ; Afonso, Rafael Sanches [2] ; Fantinatti-Garboggini, Fabiana [2] ; Arns, Clarice Weis [1]
Total Authors: 11
Affiliation:
[1] Univ Campinas UNICAMP, Inst Biol, Dept Genet & Evolut & Bioagents, Lab Virol, BR-13083970 Campinas, SP - Brazil
[2] Univ Campinas UNICAMP, Multidisciplinary Ctr Chem Biol & Agr Res, BR-13148218 Paulinia, SP - Brazil
[3] Univ Santa Cruz UESC, BR-45662900 Ilheus, BA - Brazil
[4] Univ Feira Santana UEFS, BR-44036900 Feira De Santana, BA - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Evidence-based Complementary and Alternative Medicine; 2015.
Web of Science Citations: 3
Abstract

Extracts from termite-associated bacteria were evaluated for in vitro antiviral activity against bovine viral diarrhea virus (BVDV). Two bacterial strains were identified as active, with percentages of inhibition (IP) equal to 98%. Both strains were subjected to functional analysis via the addition of virus and extract at different time points in cell culture; the results showed that they were effective as posttreatments. Moreover, we performed MTT colorimetric assays to identify the CC50, IC50, and SI values of these strains, and strain CDPA27 was considered the most promising. In parallel, the isolates were identified as Streptomyces through 16S rRNA gene sequencing analysis. Specifically, CDPA27 was identified as S. chartreusis. The CDPA27 extract was fractionated on a C18-E SPE cartridge, and the fractions were reevaluated. A 100% methanol fraction was identified to contain the compound(s) responsible for antiviral activity, which had an SI of 262.41. GC-MS analysis showed that this activity was likely associated with the compound(s) that had a peak retention time of 5 min. Taken together, the results of the present study provide new information for antiviral research using natural sources, demonstrate the antiviral potential of Streptomyces chartreusis compounds isolated from termite mounds against BVDV, and lay the foundation for further studies on the treatment of HCV infection. (AU)