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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Immunomodulatory activity of Tityus serrulatus scorpion venom on human T lymphocytes

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Casella-Martins, Andrea [1] ; Ayres, Lorena R. [1, 2] ; Burin, Sandra M. [1] ; Morais, Fabiana R. [1] ; Pereira, Juliana C. [1] ; Faccioli, Lucia H. [1] ; Sampaio, Suely V. [1] ; Arantes, Eliane C. [3] ; Castro, Fabiola A. [1] ; Pereira-Crott, Luciana S. [1]
Total Authors: 10
[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Clin Anal Toxicol & Food Sci, BR-14040903 Ribeirao Preto, SP - Brazil
[2] Univ Fed Espirito Santo, Dept Pharmaceut Sci, Vitoria, ES - Brazil
[3] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Phys & Chem, BR-14040903 Ribeirao Preto, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Journal of Venomous Animals and Toxins including Tropical Diseases; v. 21, NOV 11 2015.
Web of Science Citations: 8

Background: Tityus serrulatus scorpion venom (TsV) contains toxins that act on K+ and Na+ channels and account for the venom's toxic effects. TsV can activate murine peritoneal macrophages, but its effects on human lymphocytes have been poorly investigated. Considering that lymphocytes may play an important role in envenomation, we assessed whether TsV affects the expression of phenotypic (CD3, CD4, and CD8) and activation (CD69, CD25, and HLA-DR) markers, cell proliferation, and cytokine production in peripheral blood mononuclear cells. Methods: Cytotoxicity of TsV was evaluated via the MTT assay. Cell proliferation, expression of phenotypic and activation markers, and release of cytokines were assessed using flow cytometry, after treatment with non-cytotoxic concentrations of TsV. The combined use of carboxyfluorescein diacetate succinimidyl ester and monoclonal antibodies against phenotypic and activation markers enabled us to simultaneously assess cell proliferation extent and cell activation status, and to discriminate among cell subpopulations. Results: TsV at concentrations of 25 to 100 mu g/mL were not cytotoxic towards peripheral blood mononuclear cells. TsV did not induce significant changes in lymphocyte subpopulations or in the expression of activation markers on CD4(+) and CD8(+) T cells. TsV inhibited the phytohemagglutinin-stimulated lymphocyte proliferation, particularly in the CD8(+) CD25(+) T lymphocyte subset. TsV alone, at 50 and 100 mu g/ mL, did not induce peripheral blood mononuclear cell proliferation, but elicited the production and release of IL-6, a proinflammatory cytokine that plays an important role in innate and adaptive immune responses. Conclusions: TsV is a potential source of molecules with immunomodulatory action on human T lymphocytes. (AU)

FAPESP's process: 11/23236-4 - Native and recombinant animal toxins: functional, structural and molecular analysis
Grantee:Suely Vilela
Support type: Research Projects - Thematic Grants