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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Increased Susceptibility of Gracilinanus microtarsus Liver Mitochondria to Ca2+-Induced Permeability Transition Is Associated with a More Oxidized State of NAD(P)

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Ronchi, Juliana A. [1] ; Henning, Barbara [2] ; Ravagnani, Felipe G. [1] ; Figueira, Tiago R. [1] ; Castilho, Roger F. [1] ; Dos Reis, Sergio F. [3] ; Vercesi, Anibal E. [1]
Total Authors: 7
[1] Univ Estadual Campinas, UNICAMP, Fac Med Sci, Dept Clin Pathol, BR-13087877 Campinas, SP - Brazil
[2] Univ Estadual Campinas, Inst Biol, Grad Program Ecol, BR-13087877 Campinas, SP - Brazil
[3] Univ Estadual Campinas, Inst Biol, Dept Anim Biol, BR-13087877 Campinas, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Web of Science Citations: 0

In addition to be the cell's powerhouse, mitochondria also contain a cell death machinery that includes highly regulated processes such as the membrane permeability transition pore (PTP) and reactive oxygen species (ROS) production. In this context, the results presented here provide evidence that liver mitochondria isolated from Gracilinanus microtarsus, a small and short life span (one year) marsupial, when compared to mice, are much more susceptible to PTP opening in association with a poor NADPH dependent antioxidant capacity. Liver mitochondria isolated from the marsupial are well coupled and take up Ca2+ but exhibited a much lower Ca2+ retention capacity than mouse mitochondria. Although the known PTP inhibitors cyclosporin A, ADP, and ATP significantly increased themarsupial mitochondria capacity to retain Ca2+, their effects we remuch larger in mice than in marsupial mitochondria. Both fluorescence and HPLC analysis of mitochondrial nicotinamide nucleotides showed that both content and state of reduction (mainly of NADPH) were lower in the marsupial mitochondria than in mice mitochondria despite the similarity in the activity of the glutathione peroxidase/reductase system. Overall, these data suggest that PTP opening is an important event in processes of Ca2+ signalling to cell death mediated by mitochondrial redox imbalance in G. microtarsus . (AU)

FAPESP's process: 11/50400-0 - Mitochondrial energy metabolism, redox state and functionality in cell death and cardiometabolic and neurodegenerative disorders
Grantee:Aníbal Eugênio Vercesi
Support type: Research Projects - Thematic Grants