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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Expression Profiles of Cytokine mRNAs in the Pleural Fluid Reveal Differences Among Tuberculosis, Malignancies, and Pneumonia-Exudative Pleural Effusions

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Author(s):
Alves Esposito, Danillo Lucas [1] ; Bollela, Valdes Roberto [1] ; Pereira Feitosa, Ana Luisa [1] ; Lopes da Fonseca, Benedito Antonio [2]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo, Dept Internal Med, Sch Med Ribeirao Preto, Div Infect Dis, BR-14049900 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Dept Internal Med, Sch Med Ribeirao Preto, BR-14049900 Ribeirao Preto, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Lung; v. 193, n. 6, p. 1001-1007, DEC 2015.
Web of Science Citations: 2
Abstract

Introduction Tuberculosis (TB) and malignant diseases are the most common causes of lymphocytic pleural effusion in adults. Serum and pleural fluid cytokine levels have been analyzed to help in the differential diagnosis, but with limited results. Purpose This study investigates transcription levels of selected cytokine genes in pleural effusion of patients under investigation for TB. Methods This was a prospective study that included adult patients under investigation for pleural effusion in Brazil. The expression of 19 cytokine genes was analyzed by RT-qPCR. Results The majority of cytokine-related genes expressed in pleural fluid of TB patients were similar in non-TB patients, except for RORA and RORC genes, which showed a statistically higher level in TB. All cytokines in the Th17 pattern were induced in TB patients' pleural fluid. Patients with malignant pleural effusion expressed higher levels of IFN-alpha 1, IFN-beta 1, TNF-alpha, IL-4 and IL-6, and suppression of TGF beta-1. Conclusion There is still a lot to understand about the cytokine roles in the pro- and anti-inflammatory environment of exudative pleural effusions. The data presented here showed an increased expression of Th17 pattern cytokines genes in TB patients that could be used as markers to differentiate tuberculous pleuritis from other common causes of exudative pleural effusion. (AU)

FAPESP's process: 09/17305-3 - Quantitative analyses of inflammatory cytokine messenger RNA expression in cells of pleural effusions of patients with probable pleural tuberculosis
Grantee:Benedito Antônio Lopes da Fonseca
Support type: Regular Research Grants