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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Exercise training restores the cardiac microRNA-1 and-214 levels regulating Ca2+ handling after myocardial infarction

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Soares Melo, Stephano Freitas [1] ; Barauna, Valerio Garrone [2] ; Neves, Vander Jose [1] ; Fernandes, Tiago [1] ; Lara, Lucienne da Silva [3] ; Mazzotti, Diego Robles [4] ; Oliveira, Edilamar Menezes [1]
Total Authors: 7
[1] Univ Sao Paulo, Sch Phys Educ & Sport, Lab Biochem & Mol Biol Exercise, Sao Paulo - Brazil
[2] Univ Fed Espirito Santo, Hlth Sci Ctr, Lab Mol Physiol, Vitoria - Brazil
[3] Univ Fed Rio de Janeiro, Inst Biomed Sci, Rio De Janeiro - Brazil
[4] Univ Fed Sao Paulo, Dept Hlth Informat, Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Web of Science Citations: 24

Background: Impaired cardiomyocyte contractility and calcium handling are hallmarks of left ventricular contractile dysfunction. Exercise training has been used as a remarkable strategy in the treatment of heart disease. The microRNA-1, which targets sodium/calcium exchanger 1 (NCX), and microRNA-214, which targets sarcoplasmic reticulum calcium ATPase-2a (Serca2a), are involved in cardiac function regulation. Thus, the aim of this study was to evaluate the effect of exercise training on cardiac microRNA-1 and -214 expression after myocardial infarction. Methods: Wistar rats were randomized into four groups: sedentary sham (S-SHAM), sedentary infarction (S-INF), trained sham (T-SHAM), and trained infarction (T-INF). Exercise training consisted of 60 min/days, 5 days/week for 10 weeks with 3 % of body weight as overload beginning four weeks after myocardial infarction. Results: MicroRNA-1 and -214 expressions were, respectively, decreased (52 %) and increased (54 %) in the S-INF compared to the S-SHAM, while exercise training normalized the expression of these microRNAs. The microRNA targets NCX and Serca-2a protein expression were, respectively, decreased (55 %) and increased (34 %) in the T-INF group compared to the S-INF group. Conclusions: These results suggest that exercise training restores microRNA-1 and -214 expression levels and prevents change in both NCX and Serca-2a protein and gene expressions. Altogether, our data suggest a molecular mechanism to restore ventricular function after exercise training in myocardial infarction rats. (AU)

FAPESP's process: 09/18370-3 - MicroRNA expression on normotensive rats heart submitted to different aerobic training and the potential to hypertension therapy
Grantee:Edilamar Menezes de Oliveira
Support type: Regular Research Grants
FAPESP's process: 10/09438-0 - Therapeutic use of microRNA responsible for regulating cardiac contractility associated with exercise training aerobic in rats with heart failure
Grantee:Stéphano Freitas Soares Melo
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 12/04104-2 - MicroRNAs expression in the thoracic aorta of normotensive rats submitted to physical training: perspectives for identifying therapeutic targets related to hypertension
Grantee:Vander José das Neves
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 10/50048-1 - Cellular and functional bases of exercise in cardiovascular diseases
Grantee:Carlos Eduardo Negrão
Support type: Research Projects - Thematic Grants