Resistance training minimizes catabolic effects induced by sleep deprivation in rats

Author(s):Show less -

Monico-Neto, Marcos ^{[1, 2]} ; Moreira Antunes, Hanna Karen ^{[3, 2]} ; Lee, Kil Sun ^[4] ; Phillips, Stuart M. ^[5] ; de Campos Giampa, Sara Quaglia ^{[3, 2]} ; Souza, Helton de Sa ^{[1, 2]} ; Dattilo, Murilo ^{[1, 2]} ; Medeiros, Alessandra ^[3] ; de Moraes, Wilson Max ^[3] ; Tufik, Sergio ^[1] ; de Mello, Marco Tulio ^{[1, 6]}

Affiliation

^[1] Univ Fed Sao Paulo, Dept Psychobiol, Sao Paulo - Brazil

^[2] Lab Interdisciplinar Fisiol & Exercicio, Sao Paulo - Brazil

^[3] Univ Fed Sao Paulo, Dept Biosci, Santos - Brazil

^[4] Univ Fed Sao Paulo, Dept Biochem, Sao Paulo - Brazil

^[5] McMaster Univ, Dept Kinesiol, Hamilton, ON - Canada

^[6] Univ Fed Minas Gerais, Sch Phys Educ Physiotherapy & Occupat Therapy, Belo Horizonte, MG - Brazil

• Document type: Journal article
• Source: APPLIED PHYSIOLOGY NUTRITION AND METABOLISM; v. 40, n. 11, p. 1143-1150, NOV 2015.
• Web of Science Citations: 11

Abstract

Sleep deprivation (SD) can induce muscle atrophy. We aimed to investigate the changes underpinning SD-induced muscle atrophy and the impact of this condition on rats that were previously submitted to resistance training (RT). Adult male Wistar EPM-1 rats were randomly allocated into 1 of 5 groups: control, sham, SD (for 96 h), RT, and RT+SD. The major outcomes of this study were muscle fiber cross-sectional area (CSA), anabolic and catabolic hormone profiles, and the abundance of select proteins involved in muscle protein synthesis and degradation pathways. SD resulted in muscle atrophy; however, when SD was combined with RT, the reduction in muscle fiber CSA was attenuated. The levels of IGF-1 and testosterone were reduced in SD animals, and the RT+SD group had higher levels of these hormones than the SD group. Corticosterone was increased in the SD group compared with the control group, and this increase was minimized in the RT+SD group. The increases in corticosterone concentrations paralleled changes in the abundance of ubiquitinated proteins and the autophagic proteins LC3 and p62/SQSTM1, suggesting that corticosterone may trigger these changes. SD induced weight loss, but this loss was minimized in the RT+SD group. We conclude that SD induced muscle atrophy, probably because of the increased corticosterone and catabolic signal. High-intensity RT performed before SD was beneficial in containing muscle loss induced by SD. It also minimized the catabolic signal and increased synthetic activity, thereby minimizing the body's weight loss. (AU)

• FAPESP's process: 13/00152-5 - Effect of resistance exercise in cardiac and skeletal muscle of Wistar rats submitted to paradoxical sleep deprivation
• Grantee: Hanna Karen Moreira Antunes
• Support Opportunities: Regular Research Grants
• FAPESP's process: 11/15962-7 - Effects of resistance exercise on trophic and atrophic patways in skeletal muscle of rats with paradoxal sleep deprivation
• Grantee: Marcos Mônico Neto
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 98/14303-3 - Center for Sleep Studies
• Grantee: Sergio Tufik
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC