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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effect of Human Adipose Tissue Mesenchymal Stem Cells on the Regeneration of Ovine Articular Cartilage

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Author(s):
Zorzi, Alessandro R. [1, 2] ; Amstalden, Eliane M. I. [3] ; Plepis, Ana Maria G. [4] ; Martins, Virginia C. A. [4] ; Ferretti, Mario [1] ; Antonioli, Eliane [1] ; Duarte, Adriana S. S. [5] ; Luzo, Angela C. M. [5] ; Miranda, Joao B. [2]
Total Authors: 9
Affiliation:
[1] Hosp Israelita Albert Einstein, BR-05652900 Sao Paulo, SP - Brazil
[2] Univ Estadual Campinas, UNICAMP, Fac Med Sci, Dept Orthoped Surg, BR-13083887 Campinas, SP - Brazil
[3] Univ Estadual Campinas, UNICAMP, Fac Med Sci, Dept Pathol, BR-13083887 Campinas, SP - Brazil
[4] Univ Sao Paulo, Inst Quim Sao Carlos, BR-13566590 Sao Carlos, SP - Brazil
[5] Univ Estadual Campinas, UNICAMP, Hematol Hemotherapy Ctr, BR-13083878 Campinas, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 16, n. 11, p. 26813-26831, NOV 2015.
Web of Science Citations: 5
Abstract

Cell therapy is a promising approach to improve cartilage healing. Adipose tissue is an abundant and readily accessible cell source. Previous studies have demonstrated good cartilage repair results with adipose tissue mesenchymal stem cells in small animal experiments. This study aimed to examine these cells in a large animal model. Thirty knees of adult sheep were randomly allocated to three treatment groups: CELLS (scaffold seeded with human adipose tissue mesenchymal stem cells), SCAFFOLD (scaffold without cells), or EMPTY (untreated lesions). A partial thickness defect was created in the medial femoral condyle. After six months, the knees were examined according to an adaptation of the International Cartilage Repair Society (ICRS 1) score, in addition to a new Partial Thickness Model scale and the ICRS macroscopic score. All of the animals completed the follow-up period. The CELLS group presented with the highest ICRS 1 score (8.3 +/- 3.1), followed by the SCAFFOLD group (5.6 +/- 2.2) and the EMPTY group (5.2 +/- 2.4) (p = 0.033). Other scores were not significantly different. These results suggest that human adipose tissue mesenchymal stem cells promoted satisfactory cartilage repair in the ovine model. (AU)

FAPESP's process: 11/17596-8 - Articular cartilage repair with Type II collagen scaffold and adipose derived mesenchimal stem cells: an experimental sheep model study
Grantee:Joao Batista de Miranda
Support type: Regular Research Grants