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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Evaluation of the local inflammatory events induced by BpirMP, a metalloproteinase from Bothrops pirajai venom

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Bernardes, Carolina P. [1] ; Menaldo, Danilo L. [1] ; Mamede, Carla C. N. [2, 3] ; Zoccal, Karina F. [1] ; Cintra, Adelia C. O. [1] ; Faccioli, Lucia H. [1] ; Stanziola, Leonilda [2, 3] ; de Oliveira, Fabio [2, 3] ; Sampaio, Suely V. [1]
Total Authors: 9
[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, BR-14040903 Ribeirao Preto, SP - Brazil
[2] Univ Fed Uberlandia, ICBIM, Inst Ciencias Biomed, BR-38400 Uberlandia, MG - Brazil
[3] Inst Natl Ciencia & Tecnol Nanobiofarmaceut N Bio, Belo Horizonte, MG - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Molecular Immunology; v. 68, n. 2, B, p. 456-464, 2015.
Web of Science Citations: 11

In this study, we evaluated the edema and hyperalgesic response induced by BpirMP, a P-I class metalloproteinase isolated from Bothrops pirajai snake venom. The animals were injected with the metalloproteinase or sterile PBS (control group) and evaluated for 1, 2, 3, 4, 5, 6 and 24h. The intraplantar injection of BpirMP (5-50 mu g/paw) induced a dose- and time-dependent response. BpirMP (50 mu g) induced paw edema in rats rapidly, with peak response two hours after injection of the toxin. Also, BpirMP injection caused a significant reduction in the nociceptive threshold of the animals tested, with peak response three hours after injection of the toxin. The inflammatory mediators involved in these responses were assayed by pretreatment of animals with synthesis inhibitors or receptor antagonists. Peak responses were significantly reduced by pretreatment of animals with pyrilamine, a histamine receptor antagonist, sodium cromoglycate, a mast cell degranulation inhibitor and valeryl salicylate and meloxicam, cyclooxygenase inhibitors. The analysis of the peritoneal cavity exudate revealed an acute inflammatory response with recruitment of leukocytes, increased levels of total proteins, nitric oxide and the cytokines IL-6, TNF-alpha and IL-10. In conclusion, our results demonstrated that BpirMP induces inflammation mediated by mast cell degranulation, histamine, prostaglandins and cytokine production. (C) 2015 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 07/08361-1 - Functional and structural characterization of a non-hemorrhagic metalloprotease with antitumor and antimicrobial actvity isolated from Bothrops pirajai snake venom
Grantee:Carolina Petri Bernardes
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 11/23236-4 - Native and recombinant animal toxins: functional, structural and molecular analysis
Grantee:Suely Vilela
Support type: Research Projects - Thematic Grants
FAPESP's process: 05/54855-0 - Animal toxins: structure, function and biotechnological applications
Grantee:Suely Vilela
Support type: Research Projects - Thematic Grants