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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Brazilian Red Propolis Attenuates Inflammatory Signaling Cascade in LPS-Activated Macrophages

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Bueno-Silva, Bruno [1] ; Kawamoto, Dione [1] ; Ando-Suguimoto, Ellen S. [1] ; Alencar, Severino M. [2] ; Rosalen, Pedro L. [3] ; Mayer, Marcia P. A. [1]
Total Authors: 6
[1] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Sao Paulo, SP - Brazil
[2] ESALQ USP, Coll Agr Luiz de Queiroz, Piracicaba, SP - Brazil
[3] Univ Campinas UNICAMP, Dept Physiol Sci, Piracicaba Dent Sch, Piracicaba, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: PLoS One; v. 10, n. 12 DEC 14 2015.
Web of Science Citations: 17

Although previous studies suggested an anti-inflammatory property of Brazilian red propolis (BRP), the mechanisms involved in the anti-inflammatory effects of BRP and its activity on macrophages were still not elucidated. This study aimed to evaluate whether BRP attenuates the inflammatory effect of LPS on macrophages and to investigate its underlying mechanisms. BRP was added to RAW 264.7 murine macrophages after activation with LPS. NO production, cell viability, cytokines profile were evaluated. Activation of inflammatory signaling pathways and macrophage polarization were determined by RT-qPCR and Western blot. BRP at 50 mu g/ml inhibited NO production by 78% without affecting cell viability. Cd80 and Cd86 were upregulated whereas mrc1 was down regulated by BRP indicating macrophage polarization at M1. BRP attenuated the production of pro-inflammatory mediators IL-12, GM-CSF, IFN-gamma, IL-1 beta in cell supernatants although levels of TNF-alpha and IL-6 were slightly increased after BRP treatment. Levels of IL-4, IL-10 and TGF-beta were also reduced by BRP. BRP significantly reduced the up-regulation promoted by LPS of transcription of genes in inflammatory signaling (Pdk1, Pak1, Nfkb1, Mtcp1, Gsk3b, Fos and Elk1) and of Il1 beta and Il1f9 (fold-change rate >5), which were further confirmed by the inhibition of NF-kappa B and MAPK signaling pathways. Furthermore, the upstream adaptor MyD88 adaptor-like (Mal), also known as TIRAP, involved in TLR2 and TLR4 signaling, was down-regulated in BRP treated LPS-activated macrophages. Given that BRP inhibited multiple signaling pathways in macrophages involved in the inflammatory process activated by LPS, our data indicated that BRP is a noteworthy food-source for the discovery of new bioactive compounds and a potential candidate to attenuate exhacerbated inflammatory diseases. (AU)

FAPESP's process: 12/01500-4 - Assessment of effects of propolis and its bioactive compounds on the modulation of of macrophages inflammatory response
Grantee:Bruno Bueno Silva
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 12/14323-3 - Assessment of effects of propolis and its bioactive compounds on the modulation of of macrophages inflammatory response
Grantee:Marcia Pinto Alves Mayer
Support type: Regular Research Grants