| Full text | |
| Author(s): |
Ignacio, Aline
[1]
;
Fernandes, Miriam Rodriguez
[1]
;
Avila-Campos, Mario Julio
[1]
;
Nakano, Viviane
[1]
Total Authors: 4
|
| Affiliation: | [1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, BR-05508900 Sao Paulo - Brazil
Total Affiliations: 1
|
| Document type: | Journal article |
| Source: | Brazilian Journal of Microbiology; v. 46, n. 4, p. 1141-1145, OCT-DEC 2015. |
| Web of Science Citations: | 3 |
| Abstract | |
Enterotoxigenic Bacteroides fragilis (ETBF) is an important part of the human and animal intestinal microbiota and is commonly associated with diarrhea. ETBF strains produce an enterotoxin encoded by the bft gene located in the B. fragilis pathogenicity island (BfPAI). Non-enterotoxigenic B. fragilis (NTBF) strains lack the BfPAI and usually show two different genetic patterns, II and III, based on the absence or presence of a BfPAI-flanking region, respectively. The incidence of ETBF and NTBF strains in fecal samples isolated from children without acute diarrhea or any other intestinal disorders was determined. All 84 fecal samples evaluated were B. fragilis-positive by PCR, four of them harbored the bft gene, 27 contained the NTBF pattern III DNA sequence, and 52 were considered to be NTBF pattern II samples. One sample was positive for both ETBF and NTBF pattern III DNA sequences. All 19 B. fragilis strains isolated by the culture method were bft-negative, 9 belonged to pattern III and 10 to pattern II. We present an updated overview of the ETBF and NTBF incidence in the fecal microbiota of children from Sao Paulo City, Brazil. (AU) | |
| FAPESP's process: | 12/10659-7 - Evaluation of some microorganisms of the endogenous intestinal microbiota of children with normal weight, obese and overweight in school-age |
| Grantee: | Aline Ignacio Silvestre da Silva |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 09/03792-0 - Expression of Bacteroides fragilis bmeBAC efflux pump genes in Escherichia coli and characterization of the substrate profiles of the BmeABC pumps |
| Grantee: | Viviane Nakano |
| Support Opportunities: | Scholarships in Brazil - Young Researchers |