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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Resistance Risk Assessment of Spodoptera frugiperda (Lepidoptera: Noctuidae) and Diatraea saccharalis (Lepidoptera: Crambidae) to Vip3Aa20 Insecticidal Protein Expressed in Corn

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Author(s):
Bernardi, Oderlei [1] ; Bernardi, Daniel [1] ; Amado, Douglas [1] ; Sousa, Renan S. [1] ; Fatoretto, Julio [2] ; Medeiros, Fernanda C. L. [2] ; Conville, Jared [3] ; Burd, Tony [4] ; Omoto, Celso [1]
Total Authors: 9
Affiliation:
[1] Escola Super Agr Luiz de Queiroz ESALQ USP, Dept Entomol & Acarol, BR-13418900 Piracicaba, SP - Brazil
[2] Syngenta Crop Protect, BR-04795900 Sao Paulo - Brazil
[3] Syngenta Biotechnol Inc, Raleigh, NC - USA
[4] Syngenta Crop Protect, Greensboro, NC 27419 - USA
Total Affiliations: 4
Document type: Journal article
Source: Journal of Economic Entomology; v. 108, n. 6, p. 2711-2719, DEC 2015.
Web of Science Citations: 10
Abstract

Transgenic Agrisure Viptera 3 corn that expresses Cry1Ab, Vip3Aa20, and EPSPS proteins and Agrisure Viptera expressing Vip3Aa20 are used for control of Spodoptera frugiperda (J.E. Smith) and Diatraea saccharalis (F.) in Brazil. To support a resistance management program, resistance risk assessment studies were conducted to characterize the dose expression of Vip3Aa20 protein and level of control against these species. The Vip3Aa20 expression in Agrisure Viptera 3 and Agrisure Viptera decreased from V6 to V10 stage of growth. However, Vip3Aa20 expression in Agrisure Viptera 3 at V6 and V10 stages was 13- and 16-fold greater than Cry1Ab, respectively. The Vip3Aa20 expression in lyophilized tissue of Agrisure Viptera 3 and Agrisure Viptera diluted 25-fold in an artificial diet caused complete larval mortality of S. frugiperda and D. saccharalis. In contrast, lyophilized tissue of Bt11 at the same dilution does not provide complete mortality of these species. Agrisure Viptera 3 and Agrisure Viptera also caused a high level of mortality against S. frugiperda and D. saccharalis. Moreover, 100% mortality was observed for S. frugiperda larvae (neonates through fifth-instar larvae) when fed in corn with the Vip trait technology. Viptera corn achieves a high level of control against S. frugiperda and D. saccharalis providing a high dose, which is an important determination to support the refuge strategy for an effective resistance management program. (AU)

FAPESP's process: 11/22743-0 - Resistance risk assessment of Spodoptera frugiperda (J. E. Smith) and Diatraea saccharalis (Fabricius) (Lepidoptera: Noctuidae) to VIP3Aa20 protein expressed in genetically modified maize in Brazil
Grantee:Oderlei Bernardi
Support Opportunities: Scholarships in Brazil - Post-Doctoral