| Full text | |
| Author(s): |
Ribeiro, Amanda A.
[1]
;
Palomino, Zaira
[1]
;
Lima, Mercia P.
[2]
;
Souza, Leandro E.
[3]
;
Ferreira, Daniele S.
[4]
;
Pesquero, Joao B.
[4]
;
Irigoyen, Maria C.
[3]
;
Pesquero, Jorge L.
[5]
;
Casarini, Dulce E.
[1]
Total Authors: 9
|
| Affiliation: | [1] Univ Fed Sao Paulo, Div Nephrol, Dept Med, Escola Paulista Med, Sao Paulo - Brazil
[2] Univ Fed Minas Gerais, Sch Nursing, Dept Basic Nursing, Belo Horizonte, MG - Brazil
[3] Univ Sao Paulo, Sch Med, Inst Heart, BR-05508 Sao Paulo - Brazil
[4] Univ Fed Sao Paulo, Dept Biophys, Sao Paulo - Brazil
[5] Univ Fed Minas Gerais, Dept Biophys, Belo Horizonte, MG - Brazil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | JOURNAL OF THE RENIN-ANGIOTENSIN-ALDOSTERONE SYSTEM; v. 16, n. 4, p. 947-955, DEC 2015. |
| Web of Science Citations: | 3 |
| Abstract | |
Introduction: Tonin is an enzyme that is able to generate angiotensin II (Ang II) from angiotensin I (Ang I) or directly from angiotensinogen. Our goal was to characterize the renal renin-angiotensin system in transgenic mice that express rat tonin (TGM`(rTon)). Materials and methods: Mice were euthanized and the kidneys removed for analysis. Tonin activity was evaluated by radioimmunoassay and angiotensin I-converting enzyme (ACE) activity by HPLC. Tonin, ACE and angiotensin II-converting enzyme (ACE2) expression was analyzed by Western blotting. Results: Tonin activity was significantly increased in TGM`(rTon) compared to their respective wild-type (WT) littermates (1.7 0.21 vs 0.11 +/- 0.02 nmol of Ang II/min/mg of protein). Tonin activity had a strong positive correlation with tonin expression in both TGM`(rTon) and their respective wild-type littermates. The ACE activity and expression levels of 65-kDa N-domain angiotensin I-converting enzyme isoform were significantly increased in the TGM`(rTon) when compared with WT. ACE2 expression levels were statistically significantly higher in the TGM`(rTon) when compared with WT. Angiotensin 1-7 (Ang(1-7)) and Ang I levels were significantly lower in the TGM`(rTon). Conclusions: We suggest that the environment of tonin abundance may increase N-domain ACE activity liberated by a secretase able to cleave somatic ACE. (AU) | |
| FAPESP's process: | 09/03261-4 - Characterization of local renin angiotesin system in kidney and heart in transgenic mouse express rat tonin. |
| Grantee: | Amanda Aparecida Ribeiro |
| Support Opportunities: | Scholarships in Brazil - Master |