Antibacterial, antifungal and cytotoxic activities exhibited by endophytic fungi from the Brazilian marine red alga Bostrychia tenella (Ceramiales)

Author(s):Show less -

de Felicio, Rafael ^[1] ; Pavao, Gabriel B. ^[1] ; de Oliveira, Ana Ligia L. ^[1] ; Erbert, Cintia ^[1] ; Conti, Raphael ^[2] ; Pupo, Monica T. ^[2] ; Furtado, Niege A. J. C. ^[2] ; Ferreira, Elthon G. ^[3] ; Costa-Lotufo, Leticia V. ^[3] ; Young, Maria Claudia M. ^[4] ; Yakoya, Nair S. ^[5] ; Debonsi, Hasana M. ^[1]

Affiliation

^[1] Univ Sao Paulo, Nucleo Pesquisa Prod Nat & Sintet, Dept Fis & Quim, Fac Ciencias Farmaceut Ribeirao Preto, BR-14049 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Dept Ciencias Farmaceut, Fac Ciencias Farmaceut Ribeirao Preto, BR-14049 Ribeirao Preto, SP - Brazil

^[3] Univ Fed Ceara, Ctr Ciencias Saude, Dept Fisiol & Farmacol, Fortaleza, Ceara - Brazil

^[4] Secretaria Meio Ambiente Estado Sao Paulo, Inst Bot, Seca Fisiol & Bioquim Vegetal, Sao Paulo, SP - Brazil

^[5] Secretaria Meio Ambiente Estado Sao Paulo, Inst Bot, Nucleo Pesquisa Ficol, Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY; v. 25, n. 6, p. 641-650, NOV-DEC 2015.
• Web of Science Citations: 7

Abstract

Marine environment is one of the most important sources regarding natural products research. Besides, marine microorganisms have been denominated as a talented natural source for discovery of new leads. Although the association of macroalgae and fungi has been described regarding ecological issues, there is a lack of studies about marine seaweed endophytic fungi. In this context, the goal of this study was to evaluate cytotoxic, antifungal and antibacterial activities of endophytic fungi isolated from the Brazilian marine seaweed Bostrychia tenella (J.V. Lamouroux) J. Agardh (Ceramiales, Rhodophyta). Forty-five endophytic microorganism strains were isolated from B. tenella. Crude extracts and organic fractions of ten selected strains were obtained after growth in rice medium. Samples were evaluated for cytotoxicity, antifungal and antibacterial assays. Penicillium strains showed positive results in a diversity of assays, and other five strains were active in at least one test. In addition, cytochalasin D was isolated from Xylaria sp. This alga is composed of a microbiological potential, since its endophytic strains exhibited remarkable biological properties. Moreover, cytochalasin D isolation has confirmed chemical potential of marine endophytic strains. This is the first study in which cultured fungi isolates from the Brazilian macroalga B. tenella were evaluated concerning biological properties. Results corroborated that this species could be a pharmaceutical source from marine environment. Furthermore, Acremonium implicatum is being firstly described as marine endophyte and Xylaria sp., Trichoderma atroviride and Nigrospora oryzae as marine seaweed endophytes. Thus, this work reports the first study relating detailed isolation, cultivation and biological evaluation (cytotoxic, antifungal and antibacterial) of endophytes Penicillium decaturense and P. waksmanii from the Brazilian marine red alga B. tenella. We are also reporting the isolation of cytochalasin D, a known antitumor and antibiotic compound, from Xylaria sp. strain. Despite widespread prevalence in terrestrial and marine habitats, this present work describes the first occurrence of cytochalasin D as a metabolite from marine seaweed endophyte. (C) 2015 Sociedade Brasileira de Earmacognosia. Published by Elsevier Editora Ltda. All rights reserved. (AU)

• FAPESP's process: 10/17178-9 - Marine natural products: isolation and identification of unknown metabolites from endophytic fungi and cyanobacteria through chemical epigenetic elicitation and dereplication via molecular networking
• Grantee: Rafael de Felício
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 05/53808-9 - Chemical, biological and biosynthetic evaluation of species of red macroalgae of the Bostrychia (Rhodophyta, Rhodomelaceae) genus
• Grantee: Hosana Maria Debonsi
• Support Opportunities: BIOTA-FAPESP Program - Young Investigators Grants