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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Genotypic diversity, pathogenic potential and the resistance profile of Salmonella Typhimurium strains isolated from humans and food from 1983 to 2013 in Brazil

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Author(s):
Almeida, Fernanda [1] ; Cazentini Medeiros, Marta Ines [2] ; Rodrigues, Dalia dos Prazeres [3] ; Falcao, Juliana Pfrimer [1]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, BR-14049 Ribeirao Preto, SP - Brazil
[2] Adolfo Lutz Inst, Ctr Lab Reg Ribeirao Preto, Ribeirao Preto, SP - Brazil
[3] FIOCRUZ Fundacao Inst Oswaldo Cruz, Lab Enterobacterias, BR-21040360 Rio De Janeiro, RJ - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Journal of Medical Microbiology; v. 64, n. 11, p. 1395-1407, NOV 2015.
Web of Science Citations: 11
Abstract

Salmonella enterica subsp. enterica se rover Typhimurium is one of the leading serovars that causes salmonellosis worldwide. However, few studies have molecularly characterized S. Typhimurium strains in Brazil. In this study, we genotyped 92 S. Typhimurium strains isolated from humans (43) and food (49) between 1983 and 2013 in Brazil using PFGE, multiple-locus variable number of tandem repeats analysis (MLVA) and enterobacterial repetitive intergenic consensus PCR (ERIC-PCR). Moreover, we assessed the frequency of 12 virulence markers by FOR and the resistance profile against 12 antimicrobials. More than 85.8 % of the strains studied carried 11 of the virulence markers or more. Thirty-three strains (25 %) were multidrug resistant (MDR). The 92 S. Typhimurium studied were grouped by PFGE as PFGE-A, PFGE-B1 and PFGE-B2; by MLVA as MLVA-A, MLVA-B1 and MLVA-B2; and, finally, by ERIC-PCR as ERIC-A and ERIC-B. The strains isolated from humans before the mid-1990s were allocated to all clusters. The strains isolated from humans after the mid-1990s were distributed in the PFGE-B1, MLVA-B1, MLVA-B2 and ERIC-A clusters. The strains isolated from food were distributed in all clusters, except in PFGE-B2. All typing results suggested that the S. Typhimurium strains of human clinical origin isolated before the mid-1990s were genetically more diverse, which might indicate the selection of a more adapted S. Typhimurium subtype after Salmonella Enteritidis became the most prevalent serovar in Brazil. Regarding strains isolated from food, the results suggest the current circulation of more than one subtype. Furthermore, the high frequency of virulence genes and the presence of MDR strains reinforces their potential hazard for humans and the risk of their presence in foods in Brazil. (AU)

FAPESP's process: 12/19132-1 - Molecular characterization of Salmonella Typhimurium strains isolated from humans, food and animals in the Southeast and South regions of Brazil
Grantee:Juliana Pfrimer Falcão
Support type: Regular Research Grants