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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Anonymous nuclear markers reveal taxonomic incongruence and long-term disjunction in a cactus species complex with continental-island distribution in South America

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Author(s):
Perez, Manolo F. [1] ; Carstens, Bryan C. [2] ; Rodrigues, Gustavo L. [1] ; Moraes, Evandro M. [1]
Total Authors: 4
Affiliation:
[1] Univ Fed Sao Carlos, Dept Biol, Rodovia Joao Leme dos Santos Km 110, BR-18052780 Sao Paulo - Brazil
[2] Ohio State Univ, Dept Evolut Ecol & Organismal Biol, Columbus, OH 43210 - USA
Total Affiliations: 2
Document type: Journal article
Source: Molecular Phylogenetics and Evolution; v. 95, p. 11-19, FEB 2016.
Web of Science Citations: 7
Abstract

The Pilosocereus aurisetus complex consists of eight cactus species with a fragmented distribution associated to xeric enclaves within the Cerrado biome in eastern South America. The phylogeny of these species is incompletely resolved, and this instability complicates evolutionary analyses. Previous analyses based on both plastid and microsatellite markers suggested that this complex contained species with inherent phylogeographic structure, which was attributed to recent diversification and recurring range shifts. However, limitations of the molecular markers used in these analyses prevented some questions from being properly addressed. In order to better understand the relationship among these species and make a preliminary assessment of the genetic structure within them, we developed anonymous nuclear loci from pyrosequencing data of 40 individuals from four species in the P. aurisetus complex. The data obtained from these loci were used to identify genetic clusters within species, and to investigate the phylogenetic relationship among these inferred clusters using a species tree methodology. Coupled with a palaeodistributional modelling, our results reveal a deep phylogenetic and climatic disjunction between two geographic lineages. Our results highlight the importance of sampling more regions from the genome to gain better insights on the evolution of species with an intricate evolutionary history. The methodology used here provides a feasible approach to develop numerous genealogical molecular markers throughout the genome for non-model species. These data provide a more robust hypothesis for the relationship among the lineages of the P. aurisetus complex. (C) 2015 Elsevier Inc. All rights reserved. (AU)

FAPESP's process: 12/22477-0 - Next generation phylogeography of the Pilosocereus aurisetus cactus species group: hypotheses testing and markers development
Grantee:Evandro Marsola de Moraes
Support Opportunities: Regular Research Grants
FAPESP's process: 12/22943-1 - Multiloci phylogeography of PILOSOCEREUS AURISETUS group (Cactaceae)
Grantee:Manolo Fernandez Perez
Support Opportunities: Scholarships in Brazil - Doctorate