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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Large-scale mitogenomics enables insights into Schizophora (Diptera) radiation and population diversity

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Author(s):
Junqueira, Ana Carolina M. [1] ; Azeredo-Espin, Ana Maria L. [2, 3] ; Paulo, Daniel F. [2, 3] ; Marinho, Marco Antonio T. [4] ; Tomsho, Lynn P. [5] ; Drautz-Moses, Daniela I. [1] ; Purbojati, Rikky W. [1] ; Ratan, Aakrosh [6, 7] ; Schuster, Stephan C. [1]
Total Authors: 9
Affiliation:
[1] Nanyang Technol Univ, Singapore Ctr Environm Life Sci Engn, Singapore 637551 - Singapore
[2] Univ Estadual Campinas, Ctr Biol Mol & Engn Genet, BR-13083875 Campinas, SP - Brazil
[3] Univ Estadual Campinas, Dept Genet Evolucao & Bioagentes, BR-13083875 Campinas, SP - Brazil
[4] Univ Sao Paulo, Dept Biol, Fac Filosofia Ciencias & Letras Ribeirao Preto, BR-14040901 Ribeirao Preto, SP - Brazil
[5] Penn State Univ, Dept Biochem & Mol Biol, Ctr Comparat Genom & Bioinformat, University Pk, PA 16802 - USA
[6] Univ Virginia, Dept Publ Hlth Sci, Charlottesville, VA 22908 - USA
[7] Univ Virginia, Ctr Publ Hlth Genom, Charlottesville, VA 22908 - USA
Total Affiliations: 7
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 6, FEB 25 2016.
Web of Science Citations: 18
Abstract

True flies are insects of the order Diptera and encompass one of the most diverse groups of animals on Earth. Within dipterans, Schizophora represents a recent radiation of insects that was used as a model to develop a pipeline for generating complete mitogenomes using various sequencing platforms and strategies. 91 mitogenomes from 32 different species were sequenced and assembled with high fidelity, using amplicon, whole genome shotgun or single molecule sequencing approaches. Based on the novel mitogenomes, we estimate the origin of Schizophora within the Cretaceous-Paleogene (K-Pg) boundary, about 68.3 Ma. Detailed analyses of the blowfly family (Calliphoridae) place its origin at 22 Ma, concomitant with the radiation of grazing mammals. The emergence of ectoparasitism within calliphorids was dated 6.95 Ma for the screwworm fly and 2.3 Ma for the Australian sheep blowfly. Varying population histories were observed for the blowfly Chrysomya megacephala and the housefly Musca domestica samples in our dataset. Whereas blowflies (n = 50) appear to have undergone selective sweeps and/or severe bottlenecks in the New World, houseflies (n = 14) display variation among populations from different zoogeographical zones and low levels of gene flow. The reported highthroughput mitogenomics approach for insects enables new insights into schizophoran diversity and population history of flies. (AU)

FAPESP's process: 12/23200-2 - Molecular phylogenetics of Mesembrinellidae (Diptera: Oestroidea): inference of among-genera and among-species relationships, divergence time estimations and biogeographical aspects
Grantee:Marco Antonio Tonus Marinho
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 08/56769-2 - Mitochondrial genomics and molecular phylogeny of Schizophora (Diptera: Brachycera) using next generation sequencing
Grantee:Ana Carolina Martins Junqueira
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 10/09961-5 - Long-PCR amplification of the complete mitochondrial genome of the sub-order Brachycera (Diptera: Hexapoda) species: perspectives for molecular phylogeny
Grantee:Daniel Fernando Paulo
Support Opportunities: Scholarships in Brazil - Scientific Initiation