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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Quantification of piroxicam and 5 `-hydroxypiroxicam in human plasma and saliva using liquid chromatography-tandem mass spectrometry following oral administration

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Author(s):
Calvo, Adriana Maria [1] ; Santos, Gabriel Mulinari [1] ; Dionisio, Thiago Jose [1] ; Marques, Maria Paula [2] ; Brozoski, Daniel Thomas [1] ; Lanchote, Vera Lucia [2] ; Raposo Fernandes, Maria Helena [3] ; Cardoso Faria, Flavio Augusto [1] ; Santos, Carlos Ferreira [1]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, BR-17012901 Sao Paulo - Brazil
[2] Univ Sao Paulo, Fac Pharmaceut Sci Ribeirao Preto, Dept Clin Toxicol & Bromatol Anal, BR-17012901 Sao Paulo - Brazil
[3] Univ Porto, Fac Dent, Lab Bone Metab & Regenerat, Rua Campo Alegre 823, P-4100 Oporto - Portugal
Total Affiliations: 3
Document type: Journal article
Source: Journal of Pharmaceutical and Biomedical Analysis; v. 120, p. 212-220, FEB 20 2016.
Web of Science Citations: 2
Abstract

Saliva sampling used to quantify piroxicam and 5'-hydroxypiroxicam is a noninvasive and painless method when compared to sequential blood sampling. For that, a rapid, selective and sensitive liquid chromatography-tandem mass spectrometric method for simultaneous determination of piroxicam and 5'-hydroxypiroxicam in saliva and human plasma was developed and validated. Piroxicam and its major metabolite were separated using a LiChroCART 125-4 RP Select-B Sorbent C18 column using a mixture of methanol and 2% phosphoric acid (pH 2.7) (70:30, v/v) for the mobile phase with a flow injection of 1 mL/min. The run time was 4 min. Volunteers had saliva and blood sampled before, 1, 2, 3, 4, 5, 6, 8, 11, 24, 48 and 72 h after taking a 20 mg oral dose of piroxicam. The pharmacokinetic parameters of piroxicam in plasma samples were as follows: AUC(0-72) (64819 h ng/mL), predicted clearance (0.2 L/h), distribution volume (14.8 L), elimination half-life (50.7 h) and saliva/plasma concentration ratio (0.003). The{''}estimation of all pharmacokinetic parameters for 5'-hydroxypiroxicam would require collections beyond 72 h; however, it was possible to quantify the mean maximum concentration (133 ng/mL), time to peak concentration (53.6 h), mean AUC(0-72) (6213 h ng/mL), predicted clearance (110.31.1h) and saliva/plasma concentration ratio (0.04). The developed methods proved effective and sensitive for determining the lower quantification limit of piroxicam in plasma (6.1 ng/mL) and saliva (0.15 ng/mL) and of 5'-hydroxypiroxicam in plasma (1.2 ng/mL) and saliva (0.15 ng/mL). (C) 2015 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 09/17851-8 - Influence of cytochrome P450 (CYP2C9) genotype on the clinical efficacy and pharmacokinetics of piroxicam after lower third molar surgery
Grantee:Adriana Maria Calvo
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 14/17803-1 - Influence of genotype of cytochrome P450 (CYP2C9) on the clinical efficacy and pharmacokinetics of Piroxicam after lower third molar surgery
Grantee:Carlos Ferreira dos Santos
Support type: Regular Research Grants
FAPESP's process: 15/10198-8 - Study of the pharmacogenetics of the cytochrome p450 and pharmacokinetics of piroxicam in saliva samples by mass spectrometry
Grantee:Gabriel Mulinari dos Santos
Support type: Scholarships in Brazil - Scientific Initiation