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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Plasma Steroid Dynamics in Late- and Near-term Naturally and Artificially Conceived Bovine Pregnancies as Elucidated by Multihormone High-resolution LC-MS/MS

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Author(s):
Martins-Junior, Helio A. [1, 2] ; Pinaffi, Fabio L. V. [3] ; Simas, Rosineide C. [1, 4] ; Tarouco, Adriana K. [5, 6] ; Ferreira, Christina R. [1] ; Silva, Luciano A. [3] ; Nogueira, Guilherme P. [4] ; Meirelles, Flavio V. [5] ; Eberlin, Marcos N. [1] ; Perecin, Felipe [5]
Total Authors: 10
Affiliation:
[1] Univ Estadual Campinas, Inst Chem, ThoMSon Mass Spectrometry Lab, Campinas, SP - Brazil
[2] AB SCIEX Brazil, BR-04719002 Sao Paulo - Brazil
[3] Univ Sao Paulo, Lab Theriogenol Dr OJ Ginther, Dept Vet Med, Sch Anim Sci & Food Engn FZEA, BR-13635900 Pirassununga, SP - Brazil
[4] Sao Paulo State Univ, Dept Prod & Anim Hlth, Sch Vet Med, BR-16050680 Aracatuba, SP - Brazil
[5] Univ Sao Paulo, Lab Mol Morphophysiol & Dev, Dept Vet Med, Sch Anim Sci & Food Engn, BR-13635900 Pirassununga, SP - Brazil
[6] State Fdn Agr Res, Res Ctr Iwar Beckman, BR-96400970 Hulha Negra, RS - Brazil
Total Affiliations: 6
Document type: Journal article
Source: Endocrinology; v. 155, n. 12, p. 5011-5023, DEC 2014.
Web of Science Citations: 2
Abstract

The plasma levels of corticosteroids and sex steroids during pregnancy are key indicators of mammalian placental function and the onset of parturition. Steroid hormones are believed to be disturbed in pregnancies produced using assisted reproductive technologies (ARTs) due to placental dysfunction and the frequently observed lack of parturition signals. To elucidate the plasma steroid dynamics, a liquid chromatography-tandem mass spectrometry method was developed and used to determine the levels of corticosteroids (corticosterone, 11-deoxycortisol, and cortisol) and their direct precursors (progesterone and 17 alpha-OH-progesterone) as well as sex steroids (androstenedione, estrone, estrone sulfate, testosterone, and 17 beta-estradiol) in bovine plasma. The levels of these 10 steroids in recipient cows carrying naturally conceived (control), in vitro fertilized (IVF), or cloned (somatic cell nuclear transfer) conceptuses were compared during late-term pregnancy (30 days before parturition), during near-term pregnancy (1 day before parturition), and on the day of parturition (day 0). Significant differences were observed among the corticosteroid levels: higher levels of corticosterone, 11-deoxycortisol, and cortisol were detected in cloned pregnancies at day 30; lower levels of corticosterone were observed in ART-derived pregnancies at days 1 and 0; and estrone and estradiol levels were higher in IVF pregnancies through out the final development. These results suggested an upregulation of the P450(C11) and P450(C21) enzymes 30 days before parturition in somatic cell nuclear transfer pregnancies and an over-activation of the aromatase enzyme in IVF pregnancies. Taken together, the monitoring of multiple steroid hormones revealed that the pregnancies obtained using ART exhibited plasma steroid concentration dynamics compatible with the dysregulation of steroidogenic tissues. (AU)

FAPESP's process: 10/51677-2 - Development of new mass spectrometry techniques and their general applications in science: chemistry, biochemistry, material sciences, forensics, medicine, food science, pharmaceutical and veterinary medicine
Grantee:Marcos Nogueira Eberlin
Support type: Research Projects - Thematic Grants
FAPESP's process: 10/05806-5 - Pregnancy of bovine clones and the enigma of the preeclampsia syndrome: development of an experimental model to study vascular disorders in the uterus and placenta
Grantee:Luciano Andrade Silva
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 10/15167-0 - Development and Analytical Validation Method of Simultaneous Quantification of Estradiol and Progesterone for LC-MS/MS in Serum/Bovine Blood Plasma
Grantee:Rosineide Costa Simas
Support type: Scholarships in Brazil - Post-Doctorate