| Full text | |
| Author(s): |
Castilho, A. C. S.
[1]
;
Nogueira, M. F. G.
[2]
;
Fontes, P. K.
[1]
;
Machado, M. F.
[1]
;
Satrapa, R. A.
[1]
;
Razza, E. M.
[1]
;
Barros, C. M.
[1]
Total Authors: 7
|
| Affiliation: | [1] Univ Sao Paulo State, Inst Biosci, Dept Pharmacol, Botucatu, SP - Brazil
[2] Univ Sao Paulo State, Dept Biol Sci, Sao Paulo - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | Theriogenology; v. 82, n. 9, p. 1199-1205, DEC 2014. |
| Web of Science Citations: | 3 |
| Abstract | |
The LH plays a key role in controlling physiological processes in the ovary acting via LH receptor (LHR). In general, the effects of LHR on the regulation of granulosa cell differentiation are mediated mainly via the Gs-protein/adenylyl cyclase/cAMP system; however, the LHR activation could also induce phospholipase C (PLC)/inositol trisphosphate (IP3) via Gq/11 system. Additionally, the expression of G-proteins (GNAS, GNAQ and GNA11) and PLC beta has been showed in bovine antral follicle, concomitant with an increase in LHR expression. To gain insight into the effects of superstimulation with FSH (P-36 protocol) or FSH combined with equine chorionic gonadotropin (eCG; P-36/eCG protocol) on the mRNA expression of proteins involved in LHR signaling in bovine granulosa cells, Nelore cows (Bos indicus) were treated with two superstimulatory protocols: P-36 protocol or P-36/eCG protocol (replacement of the FSH by eCG administration on the last day of treatment). Nonsuperstimulated cows were only submitted to estrous synchronization without ovarian superstimulation. The granulosa cells were harvested from follicles and mRNA abundance of GNAS, GNAQ, GNA11, PLCB1, PLCB, PLCB4, and adenylyl cyclase isoforms (ADCY3, ADCY4, ADCY6, ADCY8, and ADGY9) was measured by real-time reserve transcription followed by polymerase chain reaction. No differences on mRNA abundance of target genes were observed in granulosa cells of cows submitted to P-36 protocol compared with control group. However, the cows submitted to P-36/eCG protocol showed upregulation on the mRNA abundance of target genes (except ADCY8) in granulosa cells. Although the P-36 protocol did not regulate mRNA expression of the proteins involved in the signaling mechanisms of the cAMP and IP3 systems, the constant presence of GNAS, GNAQ GNA11, PLCB1, PLCB3, PLCB4, and adenylyl cyclase isoforms (ADCY3, ADCY4, ADCY6, and ADCY9) mRNA and the upregulation of these genes in granulosa cells from cows submitted to P-36/eCG protocol reinforce the participation of G(q/11)/PLC/IP3 signaling as well as Gs-protein/adenylyl cyclase/cAMP system on LHR pathways during bovine granulosa cell differentiation submitted to superstimulatory treatments. (C) 2014 Elsevier Inc. All rights reserved. (AU) | |
| FAPESP's process: | 12/23409-9 - Differential gene expression, ultrastructural analysis and evaluation of the lipid profile of in vitro produced bovine blastocysts from oocytes matured conventionally or via SPOM system |
| Grantee: | Eduardo Montanari Razza |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 12/09498-9 - Influence of breed (Nellore and Angus) and follicular population on the profile of genes differentially expressed in bovine oviduct |
| Grantee: | Patricia Kubo Fontes |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 12/10737-8 - COMPARISON BETWEEN THE CONVENTIONAL IN VITRO MATURATION AND THE SIMULATION OF OOCYTE PHYSIOLOGICAL MATURATION (SPOM) IN BOVINE CUMULUS-OOCYTE COMPLEX |
| Grantee: | Mariana Fernandes Machado |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |