Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Progression of Osteogenic Cell Cultures Grown on Microtopographic Titanium Coated With Calcium Phosphate and Functionalized With a Type I Collagen-Derived Peptide

Full text
Author(s):
Show less -
Pereira, Karina K. Y. [1] ; Alves, Olivia C. [1] ; Novaes, Jr., Arthur B. [1] ; de Oliveira, Fabiola S. [1] ; Yi, Ji-Hyun [2] ; Zaniquelli, Osvaldo [1] ; Wolf-Brandstetter, Cornelia [3] ; Scharnweber, Dieter [3] ; Variola, Fabio [4] ; Nanci, Antonio [2] ; Rosa, Adalberto L. [1] ; de Oliveira, Paulo T. [1]
Total Authors: 12
Affiliation:
[1] Univ Sao Paulo, Sch Dent Ribeirao Preto, BR-14040904 Sao Paulo - Brazil
[2] Univ Montreal, Lab Study Calcified Tissues & Biomat, Montreal, PQ - Canada
[3] Tech Univ Dresden, Inst Mat Sci, Max Bergmann Ctr Biomat, Dresden - Germany
[4] Univ Ottawa, Fac Engn, Ottawa, ON - Canada
Total Affiliations: 4
Document type: Journal article
Source: Journal of Periodontology; v. 84, n. 8, p. 1199-1210, AUG 2013.
Web of Science Citations: 8
Abstract

Background: The functionalization of metallic surfaces aims at promoting the cellular response at the biomaterial-tissue interface. This study investigates the effects of the functionalization of titanium (Ti) microtopography with a calcium phosphate (CaP) coating with and without peptide 15 (P-15), a synthetic peptide analog of the cell-binding domain of collagen I, on the in vitro progression of osteogenic cells. Methods: Sandblasting and acid etching (SBAE; control) Ti microtopography was coated with CaP, enabling the loading of two concentrations of P-15: 20 or 200 mg/mL. A machined Ti was also examined. Rat calvarial osteogenic cells were cultured on Ti disks with the surfaces mentioned above for periods up to 21 days (n = 180 per group). Results: CaP coating exhibited a submicron-scale needle-shaped structure. Although all surfaces were hydrophobic at time zero, functionalization increased hydrophilicity at equilibrium. Microtopographies exhibited a lower proportion of well-spread cells at 4 hours of culture and cells with long cytoplasmic extensions at day 3; modified SBAE supported higher cell viability and larger extracellular osteopontin (OPN) accumulation. For SBAE and modified SBAE, real-time polymerase chain reaction showed the following results: 1) lower levels for runt-related transcription factor 2 at 7 days and for bone sialoprotein at days 7 and 10 as well as higher OPN levels at days 7 and 10 compared to machined Ti; and 2) higher alkaline phosphatase levels at day 10 compared to day 7. At 14 and 21 days, modified SBAE supported higher proportions of red-dye-stained areas (calcium content). Conclusion: Addition of a CaP coating to SBAE Ti by itself may affect key events of in vitro osteogenesis, ultimately resulting in enhanced matrix mineralization; additional P-15 functionalization has only limited synergistic effects. (AU)