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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Regulation of cardiac microRNAs induced by aerobic exercise training during heart failure

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Souza, Rodrigo W. A. [1] ; Fernandez, Geysson J. [1] ; Cunha, Joao P. Q. [1] ; Piedade, Warlen P. [1] ; Soares, Luana C. [1] ; Souza, Paula A. T. [1] ; de Campos, Dijon H. S. [2] ; Okoshi, Katashi [2] ; Cicogna, Antonio C. [2] ; Dal-Pai-Silva, Maeli [1] ; Carvalho, Robson F. [1]
Total Authors: 11
[1] Sao Paulo State Univ, Dept Morphol, BR-18618970 Botucatu, SP - Brazil
[2] Sao Paulo State Univ, Dept Internal Med, BR-18618970 Botucatu, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Web of Science Citations: 17

Exercise training (ET) has beneficial effects on the myocardium in heart failure (HF) patients and in animal models of induced cardiac hypertrophy and failure. We hypothesized that if microRNAs (miRNAs) respond to changes following cardiac stress, then myocardial profiling of these miRNAs may reveal cardio-protective mechanisms of aerobic ET in HF. We used ascending aortic stenosis (AS) inducing HF in Wistar rats. Controls were sham-operated animals. At 18 wk after surgery, rats with cardiac dysfunction were randomized to 10 wk of aerobic ET (HF-ET) or to a heart failure sedentary group (HF-S). ET attenuated cardiac remodeling as well as clinical and pathological signs of HF with maintenance of systolic and diastolic function when compared with that of the HF-S. Global miRNA expression profiling of the cardiac tissue revealed 53 miRNAs exclusively dysregulated in animals in the HF-ET, but only 11 miRNAs were exclusively dysregulated in the HF-S. Out of 23 miRNAs that were differentially regulated in both groups, 17 miRNAs exhibited particularly high increases in expression, including miR-598, miR-429, miR-224, miR-425, and miR-221. From the initial set of deregulated miRNAs, 14 miRNAs with validated targets expressed in cardiac tissue that respond robustly to ET in HF were used to construct miRNA-mRNA regulatory networks that revealed a set of 203 miRNA-target genes involved in programmed cell death, TGF-beta signaling, cellular metabolic processes, cytokine signaling, and cell morphogenesis. Our findings reveal that ET attenuates cardiac abnormalities during HF by regulating cardiac miRNAs with a potential role in cardio-protective mechanisms through multiple effects on gene expression. (AU)

FAPESP's process: 10/06281-3 - Micro-RNAs global expression profile in skeletal muscle of rats with heart failure
Grantee:Robson Francisco Carvalho
Support type: Regular Research Grants
FAPESP's process: 10/10583-5 - Molecular mechanisms involved in the skeletal muscle alterations in rats with heart failure submitted to aerobic training
Grantee:Maeli Dal Pai
Support type: Regular Research Grants