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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Molecular phylogeny of Toxoplasmatinae: comparison between inferences based on mitochondrial and apicoplast genetic sequences

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Author(s):
Sercundes, Michelle Klein [1] ; Oshiro Branco Valadas, Samantha Yuri [1] ; Keid, Lara Borges [2] ; Ferreira Souza Oliveira, Tricia Maria [2] ; Ferreira, Helena Lage [2] ; de Almeida Vitor, Ricardo Wagner [3] ; Gregori, Fabio [1] ; Soares, Rodrigo Martins [1]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Med Vet Prevent & Saude Anim, Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Fac Zootecnia & Engn Alimentos, Dept Med Vet, Pirassununga, SP - Brazil
[3] Univ Fed Minas Gerais, Dept Parasitol, Belo Horizonte, MG - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Revista Brasileira de Parasitologia Veterinária; v. 25, n. 1, p. 82-89, JAN-MAR 2016.
Web of Science Citations: 2
Abstract

Phylogenies within Toxoplasmatinae have been widely investigated with different molecular markers. Here, we studied molecular phylogenies of the Toxoplasmatinae subfamily based on apicoplast and mitochondrial genes. Partial sequences of apicoplast genes coding for caseinolytic protease (clpC) and beta subunit of RNA polymerase (rpoB), and mitochondrial gene coding for cytochrome B (cytB) were analyzed. Laboratory-adapted strains of the closely related parasites Sarcocystis falcatula and Sarcocystis neurona were investigated, along with Neospora caninum, Neospora hughesi, Toxoplasma gondii (strains RH, CTG and PTG), Besnoitia akodoni, Hammondia hammondi and two genetically divergent lineages of Hammondia heydorni. The molecular analysis based on organellar genes did not clearly differentiate between N. caninum and N. hughesi, but the two lineages of H. heydorni were confirmed. Slight differences between the strains of S. falcatula and S. neurona were encountered in all markers. In conclusion, congruent phylogenies were inferred from the three different genes and they might be used for screening undescribed sarcocystid parasites in order to ascertain their phylogenetic relationships with organisms of the family Sarcocystidae. The evolutionary studies based on organelar genes confirm that the genus Hammondia is paraphyletic. The primers used for amplification of clpC and rpoB were able to amplify genetic sequences of organisms of the genus Sarcocystis and organisms of the subfamily Toxoplasmatinae as well. (AU)

FAPESP's process: 11/10550-2 - Molecular characterization of Sarcocystis spp. isolates from marsupials of the genus Didelphis spp. through the analysis of mitocondrial and apicoplast genes, Internal Transcribed Spacers (ITS-1) and surface antigen genes
Grantee:Samantha Yuri Oshiro Branco Valadas
Support type: Scholarships in Brazil - Doctorate (Direct)