Calcium carbonate hybrid coating promotes the formation of biomimetic hydroxyapatite on titanium surfaces

Author(s)

Cruz, Marcos Antonio E. ^[1] ; Ruiz, Gilia C. M. ^[1] ; Faria, Amanda N. ^{[1, 2]} ; Zancanela, Daniela C. ^[1] ; Pereira, Lourivaldo S. ^[1] ; Ciancaglini, Pietro ^[1] ; Ramos, Ana P. ^[1]

Affiliation

^[1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, BR-14040901 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Bioquim & Imunol, Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: Applied Surface Science; v. 370, p. 459-468, MAY 1 2016.
• Web of Science Citations: 20

Abstract

CaCO3 particles dispersed in liquid media have proven to be good inductors of hydroxyapatite (HAp) growth. However, the use of CaCO3 deposited as thin films for this propose is unknown. Here, we report the growth of CaCO3 continuous films on Langmuir-Blodgett (LB) modified titanium surfaces and its use as HAp growth inductor. The Ti surfaces were modified with two, four, and six layers of dihexadecylphosphate (DHP)-LB films containing Ca2+, exposed to CO2 (g) for 12 h. The modified surfaces were immersed in simulated body fluid (SBF) at 37 degrees C for 36 h and submitted to bioactivity studies. This procedure originates bioactive coatings composed by non-stoichiometric HAp as evidenced by Fourier Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), and X-ray Photoelectron Spectroscopy (XPS). The presence of the CaCO3 film as pre-coating diminished the time necessary to growth continuous and homogeneous HAp films using a biomimetic approach. The surface properties of the films regarding their roughness, composition, charge, wettability, and surface free energy (gamma(s)) were accessed. The presence of HAp increased the wettability and gamma(s) of the surfaces. The coatings are not toxic for osteoblasts as observed for cell viability assays obtained after 7 and 14 days of culture. Moreover, the CaCO3 thin films promote the recovery of the osteoblasts viability more than the Ti surfaces themselves. (C) 2016 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 12/22689-8 - Modification of metallic surfaces with Langmuir-Blodgett and self-assembled polymeric films containing calcium carbonate aiming application as osteoinductive systems
• Grantee: Marcos Antonio Eufrásio Cruz
• Support type: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 12/20946-3 - Study of the growth of calcium phosphates and carbonates on organic matrices deposited on mettalic supports: obtainment of osteoinductive biomaterials
• Grantee: Ana Paula Ramos
• Support type: Regular Research Grants