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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Differential DNA Methylation of MicroRNA Genes in Temporal Cortex from Alzheimer's Disease Individuals

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Author(s):
Villela, Darine [1] ; Ramalho, Rodrigo F. [2] ; Silva, Aderbal R. T. [2] ; Brentani, Helena [3] ; Suemoto, Claudia K. [4, 5] ; Pasqualucci, Carlos Augusto [4, 6] ; Grinberg, Lea T. [4, 7] ; Krepischi, Ana C. V. [1] ; Rosenberg, Carla [1]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Dept Genet & Evolutionary Biol, Inst Biosci, Rua Matao 277, BR-05508090 Sao Paulo, SP - Brazil
[2] AC Camargo Hosp, Int Res Ctr, CIPE, Rua Tagua 440, BR-01508010 Sao Paulo, SP - Brazil
[3] Univ Sao Paulo, Sch Med, Inst & Dept Psychiat, Ave Doutor Arnaldo 455, BR-01246000 Sao Paulo, SP - Brazil
[4] Univ Sao Paulo, Sch Med, Brazilian Aging Brain Study Grp, LIM22, Dept Pathol, Ave Doutor Arnaldo 455, BR-01246000 Sao Paulo, SP - Brazil
[5] Univ Sao Paulo, Sch Med, Discipline Geriatr, Dept Internal Med, Ave Doutor Arnaldo 455, BR-01246000 Sao Paulo, SP - Brazil
[6] Univ Sao Paulo, Sch Med, Dept Pathol, Ave Doutor Arnaldo 455, BR-01246000 Sao Paulo, SP - Brazil
[7] Univ Calif San Francisco, Memory & Aging Ctr, Dept Neurol, 675 Nelson Rising Lane, POB 1207, San Francisco, CA 94143 - USA
Total Affiliations: 7
Document type: Journal article
Source: NEURAL PLASTICITY; 2016.
Web of Science Citations: 13
Abstract

This study investigated for the first time the genomewide DNA methylation changes of noncoding RNA genes in the temporal cortex samples from individuals with Alzheimer's disease (AD). The methylome of 10 AD individuals and 10 age-matched controls were obtained using Illumina 450K methylation array. A total of 2,095 among the 15,258 interrogated noncoding RNA CpG sites presented differential methylation, 161 of which were associated with miRNA genes. In particular, 10 miRNA CpG sites that were found to be hypermethylated in AD compared to control brains represent transcripts that have been previously associated with the disease. This miRNA set is predicted to target 33 coding genes from the neuregulin receptor complex (ErbB) signaling pathway, which is required for the neurons myelination process. For 6 of these miRNA genes (MIR9-1, MIR9-3, MIR181C, MIR124-1, MIR146B, and MIR451), the hypermethylation pattern is in agreement with previous results from literature that shows downregulation of miR-9, miR-181c, miR-124, miR-146b, and miR-451 in the AD brain. Our data implicate dysregulation of miRNA methylation as contributor to the pathogenesis of AD. (AU)

FAPESP's process: 10/15503-0 - Genomic imbalances on the anatomopathological and cognitive manifestations of Alzheimer’s Disease
Grantee:Darine Christina Maia Villela
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 09/00898-1 - Submicroscopic genomic imbalances associated with specific congenital abnormalities and mental deficiency phenotypes
Grantee:Carla Rosenberg
Support type: Research Projects - Thematic Grants
FAPESP's process: 13/08028-1 - CEGH-CEL - Human Genome and Stem Cell Research Center
Grantee:Mayana Zatz
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC