| Full text | |
| Author(s): |
Crevelin, Eduardo J.
[1]
;
Salami, Fernanda H.
[1]
;
Alves, Marcela N. R.
[1]
;
De Martinis, Bruno S.
[1]
;
Crotti, Antnio E. M.
[1]
;
Moraes, Luiz A. B.
[1]
Total Authors: 6
|
| Affiliation: | [1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, Ribeirao Preto, SP - Brazil
Total Affiliations: 1
|
| Document type: | Journal article |
| Source: | JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY; v. 27, n. 5, p. 944-947, MAY 2016. |
| Web of Science Citations: | 5 |
| Abstract | |
Amphetamine-type stimulants (ATS) are among illicit stimulant drugs that are most often used worldwide. A major challenge is to develop a fast and efficient methodology involving minimal sample preparation to analyze ATS in biological fluids. In this study, a urine pool solution containing amphetamine, methamphetamine, ephedrine, sibutramine, and fenfluramine at concentrations ranging from 0.5 pg/mL to 100 ng/mL was prepared and analyzed by atmospheric solids analysis probe tandem mass spectrometry (ASAP-MS/MS) and multiple reaction monitoring (MRM). A urine sample and saliva collected from a volunteer contributor (V1) were also analyzed. The limit of detection of the tested compounds ranged between 0.002 and 0.4 ng/mL in urine samples; the signal-to-noise ratio was 5. These results demonstrated that the ASAP-MS/MS methodology is applicable for the fast detection of ATS in urine samples with great sensitivity and specificity, without the need for cleanup, preconcentration, or chromatographic separation. Thus ASAP-MS/MS could potentially be used in clinical and forensic toxicology applications. (AU) | |
| FAPESP's process: | 13/50729-7 - DESI-HPTLC-Bioautography: New strategies to rapid Identification of anti-mastotos bioactive metabolites from actinomycetes. |
| Grantee: | Luiz Alberto Beraldo de Moraes |
| Support Opportunities: | BIOTA-FAPESP Program - Regular Research Grants |
| FAPESP's process: | 13/20094-0 - Evaluation of the anti-parasitary and insecticidal activities of benzofuran derivatives, and study of their gas-phase fragmentation reactions using tandem mass spectrometry |
| Grantee: | Antônio Eduardo Miller Crotti |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 12/06931-3 - Analysis of Stimulants Amphetamines in Urine and Hair Using Desorption Electrospray Ionization (DESI), and Solid Phase Microextraction in Capillary (in - tube SPME) Techniques Coupled with Sequential Mass Spectrometry (MS/MS) |
| Grantee: | Fernanda Helena Salami |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |