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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Attenuated allergic inflammatory response in the lungs during lactation

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Ochoa-Amaya, Julieta E. [1, 2] ; Marino, Larissa P. [3] ; Tobaruela, Carla N. [1] ; Namazu, Lilian B. [1] ; Calefi, Atilio S. [1] ; Margatho, Rafael [1] ; Goncalves, Jr., Vagner [1] ; Queiroz-Hazarbassanov, Nicolle [1] ; Klein, Marianne O. [1] ; Palermo-Neto, Joao [1] ; Ligeiro de Oliveira, Ana P. [4] ; Massoco, Cristina de O. [1] ; Felicio, Luciano F. [1]
Total Authors: 13
[1] Univ Sao Paulo, Dept Patol, Fac Med Vet, Sao Paulo, SP - Brazil
[2] Univ Llanos, Programa Med Vet & Zootecnia, Fac Ciencias Agr & Recursos Nat, Villavicencio - Colombia
[3] Univ Santo Amaro, Sao Paulo, SP - Brazil
[4] Univ Nove de Julho, Programa Posgrad Biofoton Aplicada Ciencias Saude, Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Life Sciences; v. 151, p. 281-287, APR 15 2016.
Web of Science Citations: 2

Aims: To evaluate the influence of lactation on lung immune function during allergic inflammation. Main methods: Female rats, 60-90 days old, were divided into three groups: no lung allergy virgins (N group), ovalbumin (OVA)-immunized and sensitized virgins (V group), and OVA-immunized and sensitized lactating females (L group). On gestation day (GD) 10, all animals in L group received a subcutaneous injection of 0.1 OVA plus aluminum hydroxide. On GD17, the L group received a subcutaneous booster injection of 10 mu g OVA plus 10 mg aluminum hydroxide. After 7 days, an inhalatory challenge with 1% OVA was given in 15 min sessions for 3 consecutive days. Animals from the V group received the same treatment, meaning both tests and time intervals between OVA treatment and inhalatory challenge were the same as in the L group. Twenty-four hours after the last inhalation session, the animals were euthanized, and the following tests were performed: total and differential bronchoalveolar lavage (BAL) and femoral marrow lavage (FML) leukocyte counts, quantification of tumor necrosis factor alpha (TNF-alpha) and interferon gamma (IFN-gamma) levels in BAL fluid, and quantification of plasma corticosterone and catecholamine levels. Key findings: The L group presented lower BAL total leukocyte counts and decreases in the number of eosinophils and macrophages compared with the V group. They also expressed higher BAL IFN-gamma and lower plasma corticosterone levels. Plasma norepinephrine levels were higher in the L group than in the N and V groups. Significance: Lactating female rats presented less intense allergic lung inflammation. Our findings suggest that lactation may protect females from asthmatic crises. (C) 2016 Elsevier Inc. All rights reserved. (AU)

FAPESP's process: 13/01610-7 - Subsistence and reproduction: opioidergic modulation of behavioral selection during lactation
Grantee:Luciano Freitas Felicio
Support type: Regular Research Grants
FAPESP's process: 09/51886-3 - Neuroimmunomodulation: drugs, stress and cytokines on nervous, endocrine and immune systems relationships
Grantee:João Palermo Neto
Support type: Research Projects - Thematic Grants