Detection of Enterotoxigenic Potential and Determination of Clonal Profile in Staphylococcus aureus and Coagulase-Negative Staphylococci Isolated from Bovine Subclinical Mastitis in Different Brazilian States

Author(s)

Mello, Priscila Luiza ^[1] ; Moraes Riboli, Danilo Flavio ^[1] ; Pinheiro, Luiza ^{[1, 2]} ; Martins, Lisiane de Almeida ^[3] ; Vasconcelos Paiva Brito, Maria Aparecida ^[4] ; Ribeiro de Souza da Cunha, Maria de Lourdes ^[1]

Affiliation

^[1] UNESP Univ Estadual Paulista, Inst Biosci Botucatu, Dept Microbiol & Immunol, BR-18618970 Botucatu, SP - Brazil

^[2] Inst Lauro de Souza Lima, Dept Anat Pathol, BR-17034971 Bauru - Brazil

^[3] Univ Paranaense UNIPAR, BR-87502210 Umuarama - Brazil

^[4] Embrapa Dairy Cattle, BR-36038330 Juiz De Fora - Brazil

• Document type: Journal article
• Source: TOXINS; v. 8, n. 4 APR 2016.
• Web of Science Citations: 10

Abstract

Epidemiological studies have identified Staphylococcus aureus as the most common agent involved in food poisoning. However, current research highlights the importance of toxigenic coagulase-negative staphylococci (CoNS) isolated from food. The aim of this study was to characterize Staphylococcus spp. isolated from cows with bovine subclinical mastitis regarding the presence of genes responsible for the production of staphylococcal enterotoxins and of the tst-1 gene encoding toxic shock syndrome toxin 1, and to determine the clonal profile of the isolates carrying any of the genes studied. A total of 181 strains isolated in different Brazilian states, including the South, Southeast, and Northeast regions, were analyzed. The sea gene was the most frequent, which was detected in 18.2% of the isolates, followed by seb in 7.7%, sec in 14.9%, sed in 0.5%, see in 8.2%, seg in 1.6%, seh in 25.4%, sei in 6.6%, and ser in 1.6%. The sej, ses, set, and tst-1 genes were not detected in any of the isolates. The typing of the isolates by pulsed-field gel electrophoresis revealed important S. aureus and S. epidermidis clusters in different areas and the presence of enterotoxin genes in lineages isolated from animals that belong to herds located geographically close to each other. (AU)

• FAPESP's process: 12/24135-0 - Staphylococcus spp. isolated from sub clinic mastitis in different Brazilian States: Antimicrobial resistance, detection of virulence factors and identification of clonal profiling
• Grantee: Priscila Luiza Mello
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 15/01401-4 - Staphylococcus spp. isolated from sub clinic mastitis in different Brazilian States: antimicrobial resistance, detection of virulence factors and identification of clonal profiling
• Grantee: Maria de Lourdes Ribeiro de Souza da Cunha
• Support Opportunities: Regular Research Grants