Valorization of Palm Oil Industrial Waste as Feedstock for Lipase Production

Author(s)

Silveira, Erick A. ^{[1, 2]} ; Tardioli, Paulo W. ^[3] ; Farinas, Cristiane S. ^{[3, 1, 2]}

Affiliation

^[1] Univ Fed Sao Carlos, Grad Program Biotechnol, BR-13565905 Sao Carlos, SP - Brazil

^[2] Embrapa Instrumentat, Rua 15 Novembro 1452, BR-13560970 Sao Carlos, SP - Brazil

^[3] Univ Fed Sao Carlos, Grad Program Chem Engn, BR-13565905 Sao Carlos, SP - Brazil

• Document type: Journal article
• Source: Applied Biochemistry and Biotechnology; v. 179, n. 4, p. 558-571, JUN 2016.
• Web of Science Citations: 4

Abstract

The use of residues from the industrial processing of palm oil as carbon source and inducer for microbial lipase production can be a way to add value to such residues and to contribute to reduced enzyme costs. The aim of this work was to investigate the feasibility of using palm oil industrial waste as feedstock for lipase production in different cultivation systems. Evaluation was made of lipase production by a selected strain of Aspergillus niger cultivated under solid-state (SSF) and submerged fermentation (SmF). Lipase activity levels up to 15.41 IU/mL were achieved under SSF. The effects of pH and temperature on the lipase activity of the SSF extract were evaluated using statistical design methodology, and maximum activities were obtained between pH 4.0 and 6.5 and at temperatures between 37 and 55 A degrees C. This lipase presented good thermal stability up to 60 A degrees C and higher specificity towards long carbon chain substrates. The results demonstrate the potential application of palm oil industrial residues for lipase production and contribute to the technological advances needed to develop processes for industrial enzymes production. (AU)

• FAPESP's process: 13/20826-0 - Production of lipase by the combined fermentation process and its application in the modification of oils and fats
• Grantee: Erick de Abreu Silveira
• Support Opportunities: Scholarships in Brazil - Doctorate