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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Genome Sequencing and Transposon Mutagenesis of Burkholderia seminalis TC3.4.2R3 Identify Genes Contributing to Suppression of Orchid Necrosis Caused by B-gladioli

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Author(s):
Araujo, Welington L. [1] ; Creason, Allison L. [2, 3] ; Mano, Emy T. [1] ; Camargo-Neves, Aline A. [4, 1] ; Minami, Sonia N. [4] ; Chang, Jeff H. [5, 2, 3] ; Loper, Joyce E. [2, 3, 6]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Lab Mol Biol & Microbial Ecol, Sao Paulo - Brazil
[2] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 - USA
[3] Oregon State Univ, Mol & Cellular Biol Program, Corvallis, OR 97331 - USA
[4] Univ Mogi Das Cruzes, Interdisciplinary Ctr Biotechnol, Mogi Das Cruzes - Brazil
[5] Oregon State Univ, Ctr Genome Res & Biocomp, Corvallis, OR 97331 - USA
[6] USDA ARS, Hort Crops Lab, Corvallis, OR - USA
Total Affiliations: 6
Document type: Journal article
Source: MOLECULAR PLANT-MICROBE INTERACTIONS; v. 29, n. 6, p. 435-446, JUN 2016.
Web of Science Citations: 6
Abstract

From a screen of 36 plant-associated strains of Burkholderia spp., we identified 24 strains that suppressed leaf and pseudobulb necrosis of orchid caused by B. gladioli. To gain insights into the mechanisms of disease suppression, we generated a draft genome sequence from one suppressive strain, TC3.4.2R3. The genome is an estimated 7.67 megabases in size, with three replicons, two chromosomes, and the plasmid pC3. Using a combination of multilocus sequence analysis and phylogenomics, we identified TC3.4.2R3 as B. seminalis, a species within the Burkholderia cepacia complex that includes opportunistic human pathogens and environmental strains. We generated and screened a library of 3,840 transposon mutants of strain TC3.4.2R3 on orchid leaves to identify genes contributing to plant disease suppression. Twelve mutants deficient in suppression of leaf necrosis were selected and the transposon insertions were mapped to eight loci. One gene is in a wcb cluster that is related to synthesis of extracellular polysaccharide, a key determinant in bacterial-host interactions in other systems, and the other seven are highly conserved among Burkholderia spp. The fundamental information developed in this study will serve as a resource for future research aiming to identify mechanisms contributing to biological control. (AU)

FAPESP's process: 13/02124-9 - Characterization of Burkholderia sp. genes associated to biological control of pathogens
Grantee:Welington Luiz de Araújo
Support type: Scholarships abroad - Research
FAPESP's process: 12/24217-6 - Endophytic microorganisms: identification of genes related to microbial interactions
Grantee:Welington Luiz de Araújo
Support type: Regular Research Grants