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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The effect of white light on normal and malignant murine melanocytes: A link between opsins, clock genes, and melanogenesis

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de Assis, L. V. M. [1] ; Moraes, M. N. [1] ; da Silveira Cruz-Machado, S. [2] ; Castrucci, A. M. L. [1]
Total Authors: 4
[1] Univ Sao Paulo, Dept Physiol, Inst Biosci, Lab Comparat Physiol Pigmentat, R Matao, Trav 14, 101, BR-05508900 Sao Paulo - Brazil
[2] Univ Sao Paulo, Dept Physiol, Inst Biosci, Lab Chronopharmacol, R Matao, Trav 14, 101, BR-05508900 Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH; v. 1863, n. 6, A, p. 1119-1133, JUN 2016.
Web of Science Citations: 19

The skin possesses a photosensitive system comprised of opsins whose function is not fully understood, and clock genes which exert an important regulatory role in skin biology. Here, we evaluated the presence of opsins in normal (Melan-a cells) and malignant (B16-F10 cells) murine melanocytes. Both cell lines express Opn2, Opn4 - for the first time reported in these cell types - as well as S-opsin. OPN4 protein was found in a small area capping the cell nuclei of B16-F10 cells kept in constant dark (DD); twenty-four hours after the white light pulse (WLP), OPN4 was found in the cell membrane. Despite the fact that B16-F10 cells expressed less Opn2 and Opn4 than Melan-a cells, our data indicate that the malignant melanocytes exhibited increased photoresponsiveness. The clock gene machinery is also severely downregulated in B16-F10 cells as compared to Melan-a cells. Pert, Per2, and Bmal1 expression increased in B16-F10 cells in response to WLP. Although no response in clock gene expression to WLP was observed in Melan-a cells, gene correlational data suggest a minor effect of WLP. In contrast to opsins and clock genes, melanogenesis is significantly upregulated in malignant melanocytes in comparison to Melan-a cells. Tyrosinase expression increased after WLP only in B16-F10 cells; however no increase in melanin content after WLP was seen in either cell line. Our findings may prove useful in the treatment and the development of new pharmacological approaches of depigmentation diseases and skin cancer. (C) 2016 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 13/24337-4 - Clock genes modulation by UVA/blue light stimulation in normal melan-A and transformed B-16 melanocytes
Grantee:Leonardo Vinícius Monteiro de Assis
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 12/50214-4 - Biological clock setting by light and temperature: phylogenetic aspects
Grantee:Ana Maria de Lauro Castrucci
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 15/04557-5 - The activation of immune-pineal axis by high-fat diet
Grantee:Sanseray da Silveira Cruz Machado
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 14/16412-9 - Mechanisms of clock genes thermo-modulation in peripheral tissue of mammals: role of TRP channels
Grantee:Maria Nathália de Carvalho Magalhães Moraes Figueira Borges
Support Opportunities: Scholarships in Brazil - Post-Doctoral