Exposure to iBites Induces a Mixed-Type Allergic Response following Salivary Antigens Challenge in Mice

Author(s):Show less -

Barros, Michele S. ^[1] ; Gomes, Eliane ^[1] ; Gueroni, Daniele I. ^[1] ; Ramos, Anderson D. ^[1] ; Mirotti, Luciana ^[1] ; Florsheim, Esther ^[1] ; Bizzarro, Bruna ^[1] ; Lino, Ciro N. R. ^{[1, 2]} ; Maciel, Ceres ^[1] ; Lino-Dos-Santos-Franco, Adriana ^[3] ; Tavares-de-Lima, Wothan ^[4] ; Capurro, Margareth L. ^{[5, 6]} ; Russo, Momtchilo ^[1] ; Sa-Nunes, Anderson ^{[1, 6]}

Affiliation

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Immunol, Sao Paulo, SP - Brazil

^[2] Hannover Med Sch, Inst Immunol, Hannover - Germany

^[3] Univ Nove de Julho UNINOVE, Grad Program Biophoton Appl Hlth Sci, Sao Paulo, SP - Brazil

^[4] Univ Sao Paulo, Inst Biomed Sci, Dept Pharmacol, Sao Paulo, SP - Brazil

^[5] Univ Sao Paulo, Inst Biomed Sci, Dept Parasitol, Sao Paulo, SP - Brazil

^[6] Natl Council Sci & Technol Dev INCT EM CNPq, Natl Inst Sci & Technol Mol Entomol, Rio De Janeiro, RJ - Brazil

• Document type: Journal article
• Source: PLoS One; v. 11, n. 5 MAY 20 2016.
• Web of Science Citations: 1

Abstract

Classical studies have shown that Aedes aegypti salivary secretion is responsible for the sensitization to mosquito bites and many of the components present in saliva are immunogenic and capable of inducing an intense immune response. Therefore, we have characterized a murine model of adjuvant-free systemic allergy induced by natural exposure to mosquito bites. BALB/c mice were sensitized by exposure to A. aegypti mosquito bites and intranasally challenged with phosphate-buffered saline only or the mosquito's salivary gland extract (SGE). Blood, bronchoalveolar lavage (BAL) and lung were collected and evaluated for cellularity, histopathological analyses, cytokines and antibody determination. Respiratory pattern was analyzed by Penh measurements and tracheal segments were obtained to study in vitro reactivity to methacholine. BAL recovered from sensitized mice following challenge with SGE showed an increased number of eosinophils and Th2 cytokines such as IL-4, IL-5 and IL-13. Peribronchoalveolar eosinophil infiltration, mucus and collagen were also observed in lung parenchyma of sensitized mice, suggesting the development of a typical Th2 response. However, the antibody profile in serum of these mice evidenced a mixed-type response with presence of both, IgG1/IgE (Th2-related) and IgG2a (Th1-related) isotypes. In addition, changes in breathing pattern and tracheal reactivity to methacholine were not found. Taken together, our results show that A. aegypti bites trigger an atypical allergic reaction, with some classical cellular and soluble Th2 components in the lung, but also systemic Th1 and Th2 antibody isotypes and no change in either the respiratory pattern or the trachea responsiveness to agonist. (AU)

• FAPESP's process: 11/15569-3 - Characterization of the biological activity of Aedes aegypti salivary serpin AET-7393
• Grantee: Ciro Novaes Rosa Lino
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 10/18216-1 - Role of host immunity on biological fitness of Aedes aegypti mosquito vector
• Grantee: Ceres Maciel de Miranda
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 09/09892-6 - Functional immunome of Aedes aegypti saliva
• Grantee: Anderson de Sá Nunes
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 09/07208-0 - Modulation of allergic lung inflammation by adjuvants or by immunological tolerance
• Grantee: Momtchilo Russo
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 09/12247-5 - Effect of Aedes aegypti saliva on the differentiation, maturation and function of dendritic cells
• Grantee: Bruna Bizzarro
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 11/22669-4 - Immunomodulation of experimental autoimmune encephalomyelitis by salivary gland extract of Aedes aegypti
• Grantee: Anderson Daniel Ramos
• Support Opportunities: Scholarships in Brazil - Doctorate